Barbara Stella

Camilla Paoletti

Carla Divieto

Clara Mattu

Daria Nurzynska

Franca Di Meglio

Giulia Tarricone

Ilaria Andreana

Letizia Nicoletti

Silvia Arpicco

Valeria Chiono

English

PORTO@iris (Publications Open Repository TOrino - Politecnico di Torino)

18 October 2022POLITECNICO DI TORINORepository ISTITUZIONALEDirect reprogramming of human cardiac fibroblasts towards the cardiac phenotype through non-viral approaches /Paoletti, Camilla; Nicoletti, Letizia; Tarricone, Giulia; Divieto, Carla; Di Meglio, Franca; Nurzynska, Daria; Mattu, Clara;Andreana, Ilaria; Arpicco, Silvia; Stella, Barbara; Chiono, Valeria. - (2020). ((Intervento presentato al convegno 5thHealthcare & Life Science & Entrepreneurship workshop tenutosi a Pisa, Italy nel 3-4 Sept 2020,.OriginalDirect reprogramming of human cardiac fibroblasts towards the cardiac phenotype through non-viralapproachesPublisher:PublishedDOI:Terms of use:openAccessPublisher copyright(Article begins on next page)This article is made available under terms and conditions as specified in the  corresponding bibliographic description inthe repositoryAvailability:This version is available at: 11583/2872570 since: 2021-02-26T09:46:29Z5th Healthcare & Life Science & Entrepreneurship workshop Direct reprogramming of human cardiac fibroblasts towards the cardiac phenotype through non-viral approaches.   Camilla Paoletti1,2, Letizia Nicoletti1,2, Giulia Tarricone1,2, Carla Divieto3, Franca Di Meglio4, Daria  Nurzynska4, Clara Mattu1,2, Ilaria Andreana5, Silvia Arpicco5, Barbara Stella 5, Valeria Chiono1,2  1 Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Turin, Italy 2 Politecnico di Torino, Polito Biomedlab, Turin, Italy 3 Istituto Nazionale di Ricerca Metrologica, Advanced Materials Metrology and Life Science, Torino, Italy 4 University of Naples Federico II, Department of Public Health, Napoli, Italy 5 University of Turin, Department of Drug Science and Technology, Turin, Italy   Myocardial infarction (MI) is the leading cause of mortality worldwide. Direct reprogramming of cardiac fibroblasts into induced cardiomyocytes (iCMs) represents a new promising strategy for cardiac regeneration [1]. In this work, we demonstrated that non-viral transient transfection of human adult cardiac fibroblasts (AHCFs) with four miRNAs (miRcombo: miR-1, 133, 208, 499 [2]) is able to reprogram AHCFs into iCMs, and reprogramming efficiency is further enhanced by a 3D culture environment. Novel lipoplexes were also designed for safer and efficient miRNA delivery [3,4].   This project received funding from the European Research Council under the European Union's Horizon 2020 research and innovation programme grant agreement No-772168.  [1] Paoletti et al. Cells 2018. [2] Paoletti et al. Front. Bioeng. Biotechnol. 2020. [3] Lee & Paoletti et al. J. Cont. Rel. 2019. [4] Arpicco et al. Farmaco 2004.  

Direct reprogramming of human cardiac fibroblasts towards the cardiac phenotype through non-viral approaches

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Direct reprogramming of human cardiac fibroblasts towards the cardiac phenotype through non-viral approaches

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