Chye, Soi Moi

Koh, Rhun Yian

Ng, Khuen Yen

Voon, Kenny Gah Leong

Yeo, Pei Ling

Parkinson’s Diseases (PD) is a debilitating neurodegenerative disorder causing movement and neurological disabilities. Apoptotic death of the dopaminergic neurons in the substantia nigra pars compacta region of midbrain of PD patients are known to be the devastating events following the buildup of oxidative stress. MicroRNA (miRNA) is a class of small, non-coding RNA which plays important roles in regulating gene expression post-transcriptionally. Increasing evidence suggested that miRNA profile might be altered in PD. A recent investigation reported that miRNA-331-5p was significantly elevated (~ 22 folds of that in controls) in the blood plasma of PD patients. However, no study has been conducted to understand the pathological involvement of this miRNA in PD. Thus, this study aims to investigate whether levels of miRNA-331-5p in neuronal cells are affected under high oxidative stress. In this study, neurotoxin, 6-hydroxydopamine (6-OHDA) was added to SH-SY5Y cells for 6, 12, and 24 hours to induce oxidative stress. Cell viability was assessed with MTT assay whereas levels of reactive oxygen species (ROS) was quantified with DCFH-DA fluorescent probe staining. Expression of miRNA-331-5p in the cells was quantified using quantitative Real-time PCR. Result shows that 6-OHDAtreatment caused a time-dependent increased in ROS generation most pronounced in 100 and 200 µM (up to 6 folds) treatment groups. 6-OHDA treatment also resulted in a time-dependent decreased of cell viability to 7% across all concentration tested. Subsequently, miRNA expression level showed significant up-regulation with 12 and 24 hours treatment groups. This increase inmiRNA-331-5p level is correlated to the increase in ROS level and neuronal cell death. In conclusion, these findings suggest thatmiRNA-331-5p might be involved in the pathogenesis of PD as its expression is altered under elevated oxidative stress

MiRNA-331-5p expression level is altered in neuronal cell under elevated oxidative stress

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