Silver oxide nanoparticles were prepared by electrochemical reduction method which is environmental bening. The tetra butyl ammonium bromide (TBAB) used as stabilizing agent in an organic medium by optimizing current density. The synthesized silver oxide nanoparticles were characterized by using UV-Visible, FT-IR, XRD, EDS and TEM analysis techniques. The nanoparticles were tested for antibacterial activity against human pathogens like gram negative Escherichia coli (E.coli),Salmonella typhiand gram positive Staphylococcus aureus, Bacillus subtilisstrains and which was proved to be excellent