Objective: The main aim of the present research was to evaluate the antioxidant and antiproliferative activity of Indian salmon (Eleutheronema tetradactylum) protein hydrolysates by pepsin and trypsin enzyme using In vitro gastrointestinal digestion procedure.Methods: Indian salmon protein hydrolysates (ISPH) were fractionated to obtain fraction I (FI), fraction II (FII) and fraction III (FIII) of peptides with different molecular weights (MW). Further, the antioxidant activity of ISPHs was evaluated by DPPH radical scavenging, metal chelating, reducing power and lipid peroxidation assays. Moreover, In vitro antiproliferative activity of ISPHs was assessed against breast cancer cell lines MCF-7. The amino acid contents of the bioactive peptides were also determined to find the correlation between the activity of peptides and their amino acid contents.Results: All bioactive peptides showed dose-dependent antioxidant activities. The highest antioxidant activity was measured in FII which was able to quench higher levels of free radicals. In the measurement of the antiproliferative capacity of peptides, they revealed nearly similar activities at low concentration. However, the cytotoxicity of peptides was significantly increased at the high dose in which only 43.9±1.8% to 65.7±1.6% cell proliferation occurred. The results showed an absence of correlation between MW and activity of peptides since the most potent bioactive peptides in our study had MWs of 1 to 3 kDa. However, hydrophobicity and presence of special amino acids like arginine and histidine is affected the activity of peptides.Conclusion: Consequently, Indian salmon protein hydrolysates were identified as good sources of antioxidant and antiproliferative peptides which could confer both nutritional and functional properties in the food industry.Keywords: Indian salmon, Protein hydrolysates, Antioxidant, Antiproliferative, Amino aci
