The aim of the work was to examine the morphology of the bone marrow of
mice during stimulation with G-CSF. Experimental Balb C mice were daily injected
subcutaneously with 250 μg/kg b.w. G-CSF (Neupogen). After 2, 4 and 6
days of the experiment femurs were obtained for morphological study. On day 2
of the mobilization the amount of haematopoietic cells in the bone marrow
increased and dilatation of the sinusoids was observed. Only single leukocytes
were observed in the lumen of the vessels. There were numerous leukocytes in
the lumen of the sinusoids on day 4 of the mobilization. The morphology of the
bone marrow on day 6 was similar to that of the control. Mobilization of mice
with G-CSF resulted in migration of haematopoietic cells from the bone marrow
and the process is most pronounced on day 4
