0 presente estudo refere-se aos detalhes do corpo utimobranquial de Heteropneustes fossilis. A glândula está localizada no intersepto entre as cavidades pericárdicas e abdominais. A glândula geralmente consiste de um parênquimasólido o qual é composto de cordões celulares. Algumas vezes, foram vistos também folículos

Singh, Sarita

Srivastav, Ajai K.

English

Cadernos Espinosanos (E-Journal)

Bolm Zool , Univ. S. Paulo 14:1-7, 1990 (1992)ULTIMOBRANCHIAL BODY OF FRESWATER CATFISH HETEROPNEUSTESFOSSIL ISSARITA SINGH & AJAI K. SRIVASTAVDepartment of Zoology, Universityof Gorakhpur, Gorakhpur, 273 009-( India) (Recebido em 25.9.1990)RESUMO: 0 presente estudo refere-se aos detalhes do corpo u 11imobranquia1 de Heteropneustes fossiiis. A glândula está localizada no intersepto entre as cavidades pericárdicas e abdominais. A glândula geralmente consiste de um parênquima sólido o qual é composto de cordbes celulares. Algumasvezes, foram vistos também folículos.ABSTRACT: The present study deals with the histologicaldetails of the # ultimobranchial body of Heteropneustes fossiiis. The gland has been located in the interseptumbetween the pericardial and abdominal cavities. The glandusually consists of a solid parenchyma which is composed ofcell cords. Sometimes, follicles have also been seen.INTRODUCTIONThe u 1timobranchiaI body (UBB) of fishes is known to be a rich source of calcitonin (CT) (ROBERTSON, 1986) CT is a major hypocalcemic hormone in mammals, but there is considerable disagreement regarding the physiological role of this hormone in fish, as hypocalcemia (LOUW et al , 1967;CHAN et al., 1968, BRADSHAW and SUTTON, 1970; LOPEZ et al ,1971, 1976: PEIGNOUX-DEVILLE et al , 1975; WALES andBARRETT, 1983; WALES, 1984; F0UCHEREAU - PER0N et al , 1987), hypercalcemia (GL0WACKI et al , 1985; FOUCHEREAU -PER0N et al , 1987) and no effect (HAYSLETT et al., 1971;PANG, 1971, 1973; ORIMO et al., 1972; COPP and MA, 1978;YAMAUCHI et a l 1978; WANDELAAR BONGA, 1980; SRIVASTAV and SWARUP, 1980; HIRAN0 et al , 1981; FENWICK and LAM, 1988;SRIVASTAV et al., 1989) have been reported after calcitonin administration to fish. In the present study, we have reported the histological details of the UBB of Heteropneustes fossiiis.2S. Singh & A. K. SrivastavMATERIALS AND METHODSAdult fish, Heteropneustes fossil is were collected and the area adjoining the heart along with the oesophagus were extirpated and fixed in aqueous Bouin s fluid. Tissues were routinely processed in graded series of alcohols, cleared in xylene and embedded in paraffin wax. Serial sections were cut at 4-6 pm and stained with HE (hematoxy1in-eosin)RESULTSThe ultimobranchial body of H. fossilis exists in the interseptum between the pericardial and abdominal cavities (Fig 1) It is not visible with naked eyes but can be detected in the serial sections of the interseptum. The gland is enveloped by thick connective tissue sheath (Fig. i) which is penetrated by blood capillaries. Occasionally, the gland is embedded within the oesophageal musculature. Usually, there exists a single patch of UBB but sometimes two or more patches have also been observed.The UBB usually consists of a solid parenchyma which is composed of cell cords (Fig. 2) Sometimes, follicles have also been seen (Fig. 3) All the cells are alike. Their cell boundaries are indistinct When stained with H/E, the cytoplasm of these cells are noticed slightly eosinophilic. The nuclei are generally vesicular in shape (Fig. 2)DISCUSSIONIn H. fossi1 is the UBB is located in the interseptum between the pericardial and abdominal cavities. Similar position of the gland has been reported earlier by KRAWARIK (1936), SEHE (1960), OGURI (1973), TAKAGI and YAMADA (1977), YAMANE (1978), ZACCONE and L0-CASCI0 (1979), ZACCONE (1980) and SRIVASTAV (1983) However, in Notopterus natopterus (SWARUP and AHMAD, 1979) and Mystus vittatus (SWARUP and AHMAD, 1983) the gland has been noticed between the oesophagus and sinus venosus.The UBB of H. fossilis is a compact structure comprising clusters of cells. OGURI (1973 - goldfish),TAKAGI and YAMADA (1977 - crucian carp), ZACCONE and LQ-CASCIO (1979 - Mugil cephalus), ZACCONE (i960 - Symphodusocellatus) and SRIVASTAV et al., (1989 - Clariss batrachus) have also reported similar structure of the gland A follicular structure of the UBB has been reported from Squalus acanthias (CAMP, 1917), carp (SEHE, 1960), Carassius auratus (ROBERTSON, 1967), shark (COPP, 1969), SalmoUltimobranchial body of freswater catfish3gairdneri (ROBERTSON, 1969), Zebrafish (YAMANE, 1978),Notopterus notopterus (SWARUP and AHMAD, 1983) EGGERT (1938) and OGURI (1973) have reported that the cellular pattern of the UBB is changeable corresponding to the glandular activity-The UBB of H. -fossil is contains a single cell type, similar to observations that have been made by SRIVASTAV et al., (1989) on Cl arias batrachus. This is in contrast to the observations on certain other species of fishes where more than one cell types have been noticed - two cell types (in eel - PEIGNOUX - DEVILLE et al. , 1975; in Sal/no gairdneri - HOOKER et al , 1979; in Notopterus notopterus SWARUP and AHMAD, 1979; in Mystus vittatus SWARUP and AHMAD, 1983) or even three cell types (in Carassius carassius - TAKAGI and YAMADA, 1977)ACKNOWLEDGMENTSOne of us (SS) is thankful to CSIR, New Delhi for providing financial assistance for this study -REFERENCESBRADSHAW, W.N. & W.W. SUTTON (1970) The influence of the environment, reproductive activity and calcitonin on the serum calcium and phosphate in the brown bullhead ( Ictaluridae). Proc UJ. Va. Acad. Sei., 42:119-120 CAMP, W.E. (1917) The development of suprapericardial (Post branchial ultimobranchial) body in Squalus acanthias. J. Morphol. , 28:369-415 CHAN, D.K.O., I CHESTER JONES 4 R. N. SMITH (1968) The effect of mammalian calcitonin on the plasma levels of calcium and inorganic phosphate in the European eel (Anguilla anguilla). Gen. Comp. Endocrinol , 11:243-245 COPP- D.H. (1969) The ultimobranchial glands and calcium regulation. In Fish Physiology, Vol II (HOAR, W.S. & RANDALL, D.J , eds.), pp 377-398, Academic Press, New York and LondonCOPP, D.H. & S.W.Y MA (1978) Endocrine control of calcium metabolism in vertebrates. In ComparativeEndocrinology, (GAILLARD, P.J. & BOER, H.H., eds) , pp. 243-253. Elsevier/North-Holland Biomedical Press, AmsterdamEGGERT, B (1938) Der ultimobranchial Körper der Knochenfische. Z Zell Mi kr- Anat., 27:754-7634S. Singh & A. K. SrivastavFENWICK, J C . ft T J. LAM (1988) Effects of calcitonin on plasma calcium and phosphate in the mudskipper,Peri ophtha1 modem schlosseri (Teleostei), in Mater during exposure to air- Gen. Comp. Endocrinol., 70:224- 230FOUCHEREAU-PERON, M., Y ARLOT-BONNEMAINS, M.S. MOUKHTAR &G. MILHAUD (1987) Calcitonin induces hypercalcemia in grey mullet and immature freshwater and Sea-water adapted rainbow trout. Comp. Biochem. Physiol., 87A:1051-1053GLOWACKI, J., J O'SULLIVAN, M. MILLER, D.W. WILKJE & L. J. DEFTOS (1985) Calcitonin produces hypercalcemia inleopard sharks. Endocrinology 166:827-829 HAYSLETT, J P -, M . EPSTEIN, D. SPECTOR, J D. MYERS, V. H.MURDAUGH & F H. EPSTEIN (1971) Effect of calcitonin onsodium metabolism in Squalus acanthias and Angui1la rostrata. Bull Aft- Desert Is. Biol Lab. 11:33-35 HIRANO, T , S. HASEGAWA, H. YAMAUCHI & H. 0RIM0 (1981) Further studies on the absence of hypocalcémie effects of eel calcitonin in the eel, Anguilla japonica. Gen. Comp. Endocrinol., 43:42-50 HOOKER, W.H., P.J. MCMILL0N & L.G. THAETE (1979) U1timobranchial gland of the trout iSalmo gairdneri)II Fine structure. Gen. Comp. Endocrinol , 38:275-284 KRAWARIK, F (1936) Uber eine bisher unbekannte Druse ohno Ausfuhrungsgong bei den heimischen Knochenfischen. Z Microsk. Anat. Forsch., 39:555-609 LOPEZ, E., J PEIGN0UX-DEVILLE & E. BAG0T (1968) Etude histophysiologique du corps ultimobranchial dun teleosteen, Angui1 la angui1 la L. au coursd * hypercalcemia expérimentais. C.R. Acad. Sei Paris, 267 D:1531-1534LOPEZ, E., J. PEIGN0UX-DEVILLE, F LALL 1ER, E. MARTELLY & C. MILET (1976) Effects of calcitonin andultimobranchialectomy (UBX) on calcium and bone metabolism in the eel Anguilla angui1 la L. Cell Tissue Res., 20:173-186 LOPEZ, E., M.M. CHART IER-BARADUC 8< J PEIGN0UX-DEV ILLE (1971) Mise en evidence del, action de la calcitonine porcine sur1'os de la déminéralisant. C. R. Acad. Sei. Pans, 272:2600-2603 L0UW, G.N., W.S. SUTTON 8< A.D. KENNY (1967) Action of tyrocalcitonin in the teleost fish Jctalurus melas. Nature 215:888-889 0GURI, M. (1973) Seasonal histologic changes in the ultimobranchial gland of goldfish. Bull Jap. Soc Sc2 Fish., 39:851-858Ultimobranchial body of freswater catfish5ORIMO, H., T FUJITA, M. YOSHIKAWA, S. WATANABE, M. OTANI & J JINNOSUKE (1972) U1timobranchia1 calcitonin of the eel Anguilla japonica. Endocrinol Jap., 19:299-302 PANG, P.K T (1971) Calcitonin and ultimobranchia1 gland in fishes. J . Exp. Zool , 178:89-100 PANG, P.K.T (1973) Endocrine control of calcium metabolism in teleosts. Amer - Zool , 13:775-792 PE IGNOUX-DEVILLE, J , E. LOPEZ, F LALL IER, E.M. BAGOT & C. MILET (1975) Responses of the ultimobranchial body in eels (Anguilla anguilla L.) maintained in sea water and experimentally matured to injection of synthetic salmon calcitonin. Cell Tiss. Res., 164:73-83 ROBERTSON, D. R. (1967) The morphology and innervation of the ultimobranchial body in the goldfish. Anat. Rec-, 157:310ROBERTSON, D. R. (1969) Some morphological observation of the ultimobranchial gland in the rainbow trout, Salmo gairdneri. J. Anat., 105:115-127 ROBERTSON, D. R. (1986) The ultimobranchial body- In Vertebrate Endocrinology Fundamentals and Biomedical implication, Vol I (PANG, P.K.T & SCHREIBMAN, M.P.,eds), pp. 235-259 Academic Press, London SEHE, C. T (i960) Studies on the ultimobranchial bodies and thyroid gland in vertebrates-fishes and amphibians. Endocrinology, 67:671-676 SRIVASTAV, AJAI K. & K . SWARUP (1980) Serum calcium of Heteropneustes fossilis (Teleost) in response to calcitonin treatment. Nat. Acad. Sei Letters, 3:373- 37 5SRIVASTAV- S.P- (1983) Studies of the endocrine glands related to calcium regulation in Clarias batrachus. Ph. D. Thesis, University of Gorakhpur, Gorakhpur, India SRIVASTAV, S.P-, K. SWARUP, S. SINGH & AJAI K. SRIVASTAV (1989) Effects of calcitonin administration on ultimobranchial gland, Stannius corpuscles andprolactin cells in male catfish Clarias batrachus. Arch. Biol., 100:385-392 SWARUP, K . & N. AHMAD (1979) Ultimobranchial body ofNotopterus notopterus in relation to calcium and sodium rich environments. Nat. Acad. Sei. Letters, 1:239-240 SWARUP, K . & N. AHMAD (1983) Ultimobranchial body of Mystus vittatus (Bloch) in response to experimental hypercalcemia. Arch. Biol., 94:247-255 TAKAGI, I & K. YAMADA (1977) An electron microscopic studyof the ultimobranchial body of the crucian carp( Carassius carassius) . Okajimas Fol Anat. Jap., 54 -.205-2286S. Singh & A. K. SrivastavWALES, N.A.M. (1984) Vascular and renal actions of salmon calcitonin in frestwater and seawater adapted European eels {Anguilla anguilla). J. Exp. Biol., 1135381-387 WALES, N.A.M. & A. L. BARRETT (1983) Repression of sodium, chloride and calcium ions in the plasma of goldfish (Carassius auratus) and immature freshwater and seawater adapted eels {Anguilla anguilla L.) after acute administration of salmon calcitonin. J. Endocrinol , 98:257-261 WENDELAAR BONGA, S. E. (1980) Effect of synthetic salmon calcitonin and low ambient calcium on plasma calcium, ultimobranchial cells, Stannius bodies and prolactin cells in the teleost Gasterosteus aculeatus. Gen. Comp. Endocrinol , 40:99-108 YAMANE, S. (1978) Histology and fine structure of the ultimobranchial gland in the Zebrafish, Brachydanio rerio. Bull Fac. Fish. Hokkaido Univ., 29:213-221 YAMAUCHI, H., M. MATSUO, A. YOSHIDA & H. 0RIM0 (1978) Effect of eel calcitonin on serum electrolytes in the ell Anguilla japonica. Gen. Comp. Endocrinol., 34:343- 346ZACCONE, G. (1980) Histochemical identification of fish ultimobranchial cells with polypeptide hormone producing APUD cells. Acta Histoe hem. , 67:13-16 ZACCONE, G. ft P- LQ-CASCIQ (1979) Distribution of the endocrine cells of the APUD series in the ultimobranchial bodies of fish. Cell Molec Biol , 24:369-372Fig. 1 Photomicrograph of ultimobranchial body (UBB) ofH. fossilis showing its position in the interseptum between the pericardial and abdominal cavities. Note the thick connective tissue sheath (CT) wehich envelops the gland. HE x 40.Fig. 2. Ultimobranchia1 body of H. fossilis showingparenchyma which is composed of cell cords. HE x400Fig. 3 Ultimobranchial body of H. fossilis exhibitingfollicles (F) and cell cords (CC) HE x 200.Ultimobranchial body of freswater catfish

Ultimobranchial body of freshwater catfish Heteropneustes fossilis

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Ultimobranchial body of freshwater catfish Heteropneustes fossilis

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