As research studies on lipoxygenase inhibition indicate quercetin as a one of the most potent
inhibitors of this enzyme, this work presents our results concerning the inhibition of soybean lipoxygease-1 by
pure quercetin. The spectra of the mixture lipoxygenase-1 and quercetin were performed by UV-Vis spectoscopy
at the time t=0 min when the two components were mixed and after 60 min of incubation of those. For the
experiments were used three quercetin concentrations, 10μM, 50μM and 100μM. The spectra show the
formation of a new peak at λ~322 and a decrease in absorbance for peaks 1, λ~273, and 3, λ~395. This fact
indicates the formation of a new compound as a result of this reaction. The formation of this new compound
increases with the increase of the concentration of the inhibitor showing that that a greater concentration of
quercetin determines a more intense interaction between lipoxygenase and inhibitor
