Over the past several decades, there has been an increase in the number of synthetic drug molecules developed and utilized to treat various conditions. Although these synthetic drugs have proven useful, there has been growing public concern regarding the potentially negative long-term effects of synthetic agents on the body. As a result, there has been an increased interest in identifying and utilizing plant extracts and purified compounds since they are perceived to be a more natural alternative to synthetic drugs. The goal of this study was to evaluate the specific antioxidant properties of alsike clover Trifolum hybridum when produced under differing growing conditions. The alsike clover was collected from the campus of Cedarville University, Cedarville, Ohio for testing. Alsike clover was removed from the field in January 2013, and transplanted indoors under grow lights for 14 days. These plants were then subjected to three separate 60-day treatments: control treatment - watering to field capacity with no fertilizer; positive treatment - watering to field capacity with fertilizer; and negative treatment - half of the water given to the field capacity treatment with no fertilizer. The rationale for choosing these different treatments was to evaluate the effects of specific growing conditions on bioactive secondary metabolite production in alsike clover. The biological evaluation was accomplished by conducting diphenylpicrylhyrazyl (DPPH) free-radical scavenging and Folin Ciocalteu assays to assess the concentration of polyphenolic compounds. Results from these experiments indicate that the biological and chemical profiles of alsike clover can be influenced by the environmental conditions under which the plants are grown
