<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;letter-spacing:-.1pt;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">Physicochemical and chromatographic evaluation of <i>Shivakshar Pachan Churna</i></span>

Abstract

609-613<span style="font-size:11.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">In the present study, an attempt was made to formulate in-house ‘Shivakshar Pachan Churna’, (IN) which was compared with some marketed preparations. (R, Z and V) The study included determination of various physicochemical and phytochemical evaluations which was followed by Chromatographic evaluations (TLC and HPTLC) of the formulations. Further, the study included standardization of these formulations by developing a simple, precise and accurate High Performance Thin Layer Chromatography (HPTLC) method using Ferulic acid (99%) as chemical marker. Chromatographic analysis was performed on silica gel 60 F254 precoated TLC plates using Toluene: Ethyl acetate: Formic acid (14:8: 0.15 v/v/v) for Ferulic acid as mobile phase. The Rf values of Ferulic acid was the basis of confirmation of this marker. The total peak areas of standard marker and corresponding formulations were compared and their contents were estimated in all the formulations. From the results, we observed that, all the physicochemical properties were found to be within the prescribed pharmacopoeial limits. The results also demonstrated that, <span style="font-size:11.0pt; font-family:" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";="" mso-bidi-font-family:mangal;mso-ansi-language:en-in;mso-fareast-language:en-us;="" mso-bidi-language:hi;mso-bidi-font-weight:bold"="">marketed formulations Z and V had a lowest concentration of Ferulic acid, while laboratory prepared formulations contained more concentration. <span style="font-size:11.0pt; font-family:" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";="" mso-bidi-font-family:mangal;mso-ansi-language:en-in;mso-fareast-language:en-us;="" mso-bidi-language:hi"="">In conclusion, the analytical method developed herein for standardization of ‘<span style="font-size:11.0pt; font-family:" times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";="" mso-bidi-font-family:mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;="" mso-bidi-language:hi"="" lang="EN-GB">Shivakshar Pachan Churna’ will be helpful in obtaining a quality control profile of this formulation.</span