Bai, Juan

Jang, Miran

Jun-Bae, An

Kim, Gun-Hee

Kim, Kee Hong

Park, Yeonhwa

Zhang, Yuan

English

Purdue E-Pubs

Purdue University Purdue e-Pubs Department of Food Science Faculty Publications Department of Food Science 5-29-2020 Piceatannol, a Natural Stilbene, Extends the Lifespan of Caenorhabditis elegans Under Fasting Through Inhibition of Lipolysis Miran Jang Purdue University Yuan Zhang Purdue University Juan Bai Purdue University An Jun-Bae Seowon University Gun-Hee Kim Duksung Women’s University See next page for additional authors Follow this and additional works at: https://docs.lib.purdue.edu/foodscipubs Recommended Citation Jang Miran, Zhang Yuan, Bai Juan, Jun-Bae An, Park Yeonhwa, Kim Gun-Hee, Kee-Hong Kim, Piceatannol, a Natural Stilbene, Extends the Lifespan of Caenorhabditis elegans Under Fasting Through Inhibition of Lipolysis, Current Developments in Nutrition, Volume 4, Issue Supplement_2, June 2020, Page 38, https://doi.org/10.1093/cdn/nzaa040_038 This document has been made available through Purdue e-Pubs, a service of the Purdue University Libraries. Please contact epubs@purdue.edu for additional information. Authors Miran Jang, Yuan Zhang, Juan Bai, An Jun-Bae, Gun-Hee Kim, Yeonhwa Park, and Kee Hong Kim This article is available at Purdue e-Pubs: https://docs.lib.purdue.edu/foodscipubs/35 38 Aging and Chronic DiseasePiceatannol, a Natural Stilbene, Extends the Lifespan ofCaenorhabditis elegans Under Fasting Through Inhibition ofLipolysisMiran Jang1, Yuan Zhang1, Juan Bai1, An Jun-Bae2, YeonhwaPark3, Gun-Hee Kim4, and Kee-Hong Kim11Purdue University; 2Seowon University; 3University of Mas-sachusetts Amherst; and 4Duksung Women’s UniversityObjectives: Lipolysis is the catabolic process that hydrolyzestriglyceride (TG) to free fatty acids (FFAs) and glycerol under negativeenergy balance such as fasting. In adipocytes, adipose TG lipase(ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipaseplay key roles in a series of TG hydrolysis reactions in mammals.However, overly activated adipose lipolysis is believed to contributeto link between obesity and systemic inflammation and oxidativestress. We previously demonstrated that piceatannol (PIC), a naturalresveratrol analogue, inhibits adipogenesis in cultured adipocytes andlipogenesis in Caenorhabditis elegans. Furthermore, we showed thatPIC extends the lifespan of C. elegans via the insulin/IGF-1 signaling.However, the effects of PIC on lipid metabolism during fasting state isunknown.Methods: We conducted Oil-Red-O assay, Enzyme assay (TG andFree glycerol contents), PCR analysis and lifespan assay.Results: In this study, we demonstrated that PIC-treated C. elegansexhibited suppressed lipolysis under fasting as judged by increasedlipid accumulation and TG levels with decreased free glycerol level.Consistent with these findings, PIC treatment resulted in decreasedmRNA levels of genes involved lipolysis such as atgl-1, hosl-1 and aak-2 in fasted C. elegans. Also, PIC treatment augmented fasting-inducedlifespan of C. elegans by an increased daf-16 gene expression. However,such effect was abolished when atgl-1, aak-2, and daf-16 mutants weretreated with PIC. In addition, we also found that autophagy is requiredfor PIC-induced lifespan in C. elegans during fasting since autophagyinhibitor treatments and autophagy gene deficient worms resulted inblunting the lifespan extension effect of PIC.Conclusions: Collectively, our results indicate that PIC contributesto lifespan extension in C. elegans during fasting possibly throughregulating lipolysis- and/or autophagy-dependent lipid metabolism.Funding Sources: 1. The National Research Foundation ofKorea (NRF) grant funded by the Korea government (MSIP)(2019R1A2C1086146) and (2019R1A6A3A03033878) 2. The RuralDevelopment Administration of the Republic of Korea.CURRENT DEVELOPMENTS IN NUTRITIONDownloaded from https://academic.oup.com/cdn/article/4/Supplement_2/38/5844529 by Purdue University Libraries ADMN user on 21 July 2022

Piceatannol, a Natural Stilbene, Extends the Lifespan of
Caenorhabditis elegans Under Fasting Through Inhibition of
Lipolysis

https://docs.lib.purdue.edu/cgi/viewcontent.cgi?article=1034&context=foodscipubs

Piceatannol, a Natural Stilbene, Extends the Lifespan of Caenorhabditis elegans Under Fasting Through Inhibition of Lipolysis

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