Germination indices of orthodox seeds as influenced by controlled deterioration and cathodic water seed invigoration y

Abstract

© 2020 Triveni Enterprises. All rights reserved. Aim: The aim of this study was to test the effects of priming on the germination of deteriorated orthodox seeds of five plant species. Methodology: The water content of seeds were raised to 14%, and the seeds then deteriorated to 50% viability at 40oC and 100% relative humidity. Deteriorated seeds and fresh seeds of test species were then primed with cathodic water, un-electrophorized calcium magnesium solution and deionised water. Unprimed fresh and unprimed controlled deteriorated seeds served as controls. Germination indices were determined. Results: In general, all priming treatments had positive effects on the germination indices of deteriorated seeds of all species, and a lesser extent on fresh seeds. However, seeds treated with cathodic water performed better than seeds treated with either CaMg solution or deionised water. While controlled deterioration reduced DNA concentrations and the DNA purity of seeds, priming, particularly with cathodic water had a protecting effect on DNA. Priming also boosted amylase activities in both fresh and deteriorated seeds. Interpretation: Results are consistent with cathodic water reducing oxidative stress during imbibition. Cathodic water seed priming, therefore, has the potential to play a significant role in the conservation of orthodox seeds