Microwave assisted synthesis of polyaniline modified by pullulan (PANI/Pull) composite wasperformed by aniline oxidation with potassium iodate. The PANI/Pull composite was characterizedusing ATR-FTIR technique. FTIR spectra confirm presence of both components in PANI/Pullcomposite. Antimicrobial evaluation of PANI/Pull material performed by using a qualitative diskdiffusion method on Candida albicans (C. albicans) culture showed very high sensitivity toPANI/Pull composite. Observed FTIR and antifungal activity represent a promising results especiallyfor potential biomedical applications of PANI/Pull composite.Poster: [https://cherry.chem.bg.ac.rs/handle/123456789/5431

Bajuk-Bogdanović, Danica

Beškoski, Vladimir

Bubanja, Itana Nuša

Gizdavić-Nikolaidis, Marija

Gojgić-Cvijović, Gordana

Lješević, Marija

Lončarević, Branka D.

Stanisavljev, Dragomir

Gojgić-Cvijović, Gordana D.

Faculty of Chemistry Repository - Cherry

     PHYSICAL CHEMISTRY 2021  15th International Conference on Fundamental and Applied Aspects of Physical Chemistry    Proceedings Volume II                    September 20-24, 2021  Belgrade, Serbia        PHYSICAL CHEMISTRY 2021  15th International Conference on Fundamental and Applied Aspects of Physical Chemistry    Proceedings Volume II    The Conference is dedicated to the   30th Anniversary of the founding of the Society of Physical Chemists of Serbia  and  100th Anniversary of Bray-Liebhafsky reaction        September 20-24, 2021 Belgrade, Serbia  II  Title: Physical Chemistry 2021 (Proceedings) ISBN 978-86-82475-40-8 Volume II: ISBN 978-86-82475-39-2 Editors: Željko Čupić and Slobodan Anić Published by: Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia Publisher: Society of Physical Chemists of Serbia For Publisher: S. Anić, President of Society of Physical Chemists of Serbia Printed by: ʺJovanʺ, <Printing and Publishing Company, 200 Copies Number of pages: 6+388, Format A4, printing finished in December 2021  Text and Layout: ʺJovanʺ   Neither this book nor any part may be reproduced or transmitted in any form or by any means, including photocopying, or by any information storage and retrieval system, without permission in writing from the publisher.            200 - Copy printing                                III           CONTENT    Volume II  Organizer IV Comittes V Organic Physical Chemistry 345 Material Science 367 Macromolecular Physical Chemistry 487 Environmental Protection, Forensic Sciences, Geophysical Chemistry, Radiochemistry, Nuclear Chemistry 519 Phase Boundaries, Colloids, Liquid Crystals, Surface-Active Substances 633 Complex Compounds 643 General Physical Chemistry 655 Pharmaceutical Physical Chemistry 669 Food Physical Chemistry 679 Physico-Chemical Analysis 703 Index 725     IV        PHYSICAL CHEMISTRY 2021  15th International Conference on  Fundamental and Applied Aspects of Physical Chemistry     Organized by  The Society of Physical Chemists of Serbia  in co-operation with  Institute of Catalysis Bulgarian Academy of Sciences  and  Boreskov Institute of Catalysis Siberian Branch of   Russian Academy of Sciences  and  University of Belgrade, Serbia:  Faculty of Physical Chemistry Institute of Chemistry, Technology and Metallurgy Vinča Institute of Nuclear Sciences Faculty of Pharmacy  and  Institute of General and Physical Chemistry, Belgrade, Serbia        V  International Organizing Committee   Chairman:   S. Anić (Serbia) Vice-chairman: M. Gabrovska (Bulgaria)                                A. A. Vedyagin (Russia)       S. N. Blagojević (Serbia)  Members: N. Cvjetićanin (Serbia), S. M. Blagojević (Serbia), M. Daković (Serbia), J. Dimitrić Marković (Serbia), T. Grozdić (Serbia), Lj. Ignjatović (Serbia), D. Jovanović (Serbia), M. Kuzmanović (Serbia), D. Marković (Serbia), B. Milosavljević (USA), M. Mojović (Serbia), N. Pejić (Serbia), M. Petković (Serbia), A. Popović-Bjelić (Serbia), B. Simonović (Serbia), M.Stanković (Serbia), B. Šljukić (Serbia), G. Tasić (Serbia), S. Veličković (Serbia), N. Vukelić (Serbia)  International Scientific Committee  Chairman:   Ž. Čupić (Serbia) Vice-chairman: V. Bukhtiyarov (Russia) S. Todorova (Bulgaria) B. Adnađević (Serbia)  Members: S. Anić (Serbia), A. Antić-Jovanović (Serbia), A. Azizoğlu (Turky), R. Cervellati (Italy), G. Ćirić-Marjanović (Serbia), V. Dondur (Serbia), I. I. Grinvald (Russia), R. Jerala (Slovenia), M. Jeremić (Serbia), G. N. Kaluđerović (Germany), E. Kiš (Serbia), A.V. Knyazev (Russia), Lj. Kolar-Anić (Serbia), U. Kortz (Germany), T. Kowalska (Poljska), A. Lemarchand (France), G. Lente (Hungary), Z. Marković (Serbia), S. Mentus (Serbia), K. Novaković (UK), N. Ostrovski (Serbia), V. Parmon (Russia), Z. Petkova Cherkezova-Zheleva (Bulgary), M. Plavšić (Serbia), J. Savović (Serbia), G. Schmitz (Belgium), I. Schreiber (Czech), L. Schreiberova (Czech), D. Stanisavljev (Serbia), N. Stepanov (Russia), M. Stojanović (USA), E. Szabó (Slovakia), Zs. Szakacs (Romania), Z. Šaponjić (Serbia), Á. Tóth (Hungary), M. Trtica (Serbia), V. Vasić (Serbia), D. Veselinović (Serbia), V. Vukojević (Sweden)  Local Executive Committee  Chairman:   S. N. Blagojević Vice-chairman: A. Ivanović-Šašić       N. Jović-Jovičić       A. Stanojević   Members:  M. Ajduković, I. N. Bubanja, A. Dobrota, J. Dostanić, D. Dimić, S. Jovanović, Z. Jovanović, D. Lončarević, M. Kragović, J. Krstić, B. Marković, S. Maćešić, J. Maksimović, S. Marinović, D. Milenković, T. Mudrinić, M. Pagnacco, N. Potkonjak, B. Stanković, I. Stefanović, G. Stevanović, A. Stoiljković, M. Vasić       PHYSICAL CHEMISTRY 2021        H-21-P  445  MICROWAVE ASSISTED SYNTHESIS OF POLYANILINE/PULLULAN (PANI/PULL) COMPOSITE  I. N. Bubanja1, B. Lončarević2, M. Lješević2, D. Stanisavljev1, V. Beškoski3, G. Gojgić-Cvijović2, D. Bajuk-Bogdanović1 and M. Gizdavić-Nikolaidis4  1 Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia (itana.bubanja@ffh.bg.ac.rs) 2 Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, Belgrade, Serbia 3 University of Belgrade-Faculty of Chemistry, Department of Biochemistry, Studentski trg 12-15, Belgrade, Serbia  4 Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, the University of Auckland, Auckland 1023, New Zealand   ABSTRACT Microwave assisted synthesis of polyaniline modified by pullulan (PANI/Pull) composite was performed by aniline oxidation with potassium iodate. The PANI/Pull composite was characterized using ATR-FTIR technique. FTIR spectra confirm presence of both components in PANI/Pull composite. Antimicrobial evaluation of PANI/Pull material performed by using a qualitative disk diffusion method on Candida albicans (C. albicans) culture showed very high sensitivity to PANI/Pull composite. Observed FTIR and antifungal activity represent a promising results especially for potential biomedical applications of PANI/Pull composite.  INTRODUCTION Growing interest in modification of conducting polymers with biopolymers is due to the fact that a simple procedure can be used to couple the properties of both, conducting polymers and biopolymers, in order to develop materials with improved characteristics [1]. As a conducting polymer, polyaniline (PANI) has achieved great attention due to its high electrical conductivity, unique redox properties, simple preparation and doping procedure, low cost, and therefore it has found a wide range of applications [1,2]. Besides mentioned, it has been shown that PANI represents a novel antimicrobial agent that is active against a broad range of bacteria and thus has a potential applications in food safety and biomedicine as an infection control coatings [3]. The aim of our work was to modify PANI by pullulan to obtain novel and improved composite. Thanks to pullulan's biocompatibility, non-toxicity, biodegradability and wide range of applicability (as low-calorie food additive, packaging material, usage in target drug therapy, tissue engineering, wound healing etc.) [4,5] this polysaccharide biopolymer seemed to be a good candidate as a potential PANI modifier. In this paper, MW assisted copolymerization of aniline with pullulan using potassium iodate (KIO3) as an oxidant was conducted.  METHODS MW Assisted Synthesis. Microwave synthesis of PANI/Pull composite was performed in CEM reactor (CEM Discover 300-W single mode reactor operating at 2.45 GHz). Since the key part of our experiments was to keep a constant temperature as well as an irradiation power during the synthesis, it was carried out in specially designed double jacket reaction vessel which was connected to an external cooling circulating thermostat. For temperature measurements a fiber optic temperature sensor was used. Also, to achieve uniform temperature, the sample was mixed by magnetic stirring PHYSICAL CHEMISTRY 2021        H-21-P  446  at 400 rpm. Absorbed MW power, P was calculated from: P =  C ∙ m ∙ (∆T ∆t⁄ )i, where C represents heat capacity and its value is approximated with the value for pure water of 4.18 J/g°C, m is mass of the solution and (∆T ∆t⁄ )i is estimated from the linear temperature increase throughout initial heating period during which dissipation of heat by the external thermostat was small. From mentioned calorimetric method the absorbed power for 80 W emitted power, P and its standard deviation were calculated from six replicates P = (10.0 ± 0.5) W. With this experimental design the temperature of the reaction mixture before addition of aniline was maintained at (26 ± 2)°C. PANI/Pull composite was prepared by aniline oxidation with potassium iodate. In 12 mL of an aqueous solution of 1.25 M hydrochloric acid, 0.432 g KIO3 and 0.343 g pullulan (or 70 wt% relative to aniline) were added. After initial heating period and when temperature reached constant value aniline (0.480 mL or 0.490 g) was added to HCl, KIO3 and pullulan mixture. MW synthesis was carried out for 10 min. The PANI/Pull product was obtained by centrifugation. Product was washed thoroughly with 1.25 M HCl aqueous solution, deionized water and acetone to eliminate impurities and low molecular weight oligomers. The product was dried in oven at 40 °C overnight.  FTIR characterization. The ATR-FTIR spectra were recorded using Thermo Scientific Nicolet iS20 FT-IR spectrophotometer. A sample was placed in direct contact with an infrared attenuated total reflection (ATR) diamond crystal. All FTIR spectra were collected in the wavenumber range of 1800–600 cm−1 by co-adding 32 scans with a resolution of 4 cm−1. Antimicrobial activity. The qualitative antimicrobial evaluation of PANI and PANI/Pull materials was performed using a disk diffusion method [6] with reference culture of fungal strain C. albicans (ATCC 24433). Fungal inoculum was adjusted to the concentration of 1-2∙106 CFU/mL, and 100 µL were seeded in Petri dishes containing Malt agar, followed by placing 1 mg of PANI and PANI/Pull materials on top of it. The Petri plates were incubated at 28 °C for 48 h.  RESULTS AND DISCUSSION Obtained PANI/Pull composite was characterized using ATR-FTIR technique. Besides composite, pure PANI was synthetized as well under the same experimental conditions. The characteristic peaks observed in the FT-IR spectra of PANI/Pull composite give valuable information regarding to the possible interactions between pullulan and PANI. At Figure 1. the ATR-FTIR spectra of PANI (a), PANI/Pull composite (b) and pure pullulan (c) is shown. The spectrum of PANI/Pull composite exhibits bands characteristic of PANI as well as of pullulan which confirms the presence of both components in the composite. For pure PANI (Fig. l(a)), the characteristic bands [2] attributed to C-C stretching quinoid band, C-C stretching benzenoid band, C-N stretching benzenoid band, band of deprotonated PANI and out-of-plane bending vibration of C-H on para distributed ring appear at 1575, 1492, 1296, 1138 and 826 cm-1 respectively. The strongest band at 993 cm-1 in the spectrum of pullulan (Fig. l(c)) corresponds to glycosidic linkages [7]. The most prominent changes of the spectrum of the PANI/Pull composite (Fig. l(b)), in comparison to the spectrum of the pure PANI, is the red shift of peaks at 1575 and 1492 cm-1 (quinonoid (Q) and benzenoid (B) ring-stretching vibrations, respectively) to 1554 and 1458 cm-1 and the increase in absorption at about 1000 cm-1, due to the presence of pullulan during the polymerization of aniline. Also, the band at 1296 cm−1, observed in the PANI spectrum attributed to C-N stretching of secondary amine, is significantly weakened and shifted to 1287 cm–1 in the spectrum of composite. Qualitative antimicrobial evaluation of PANI and PANI/Pull materials was performed using a disk diffusion method on C. albicans culture.  PHYSICAL CHEMISTRY 2021        H-21-P  447    Figure 1. The ATR-FTIR spectra of PANI (a), PANI/Pull composite (b) and pure pullulan (c).  Even though slightly larger inhibition zone was obtained for PANI/Pull composite, obtained results suggest a high sensitivity of C. albicans to both PANI and PANI/Pull composite, indicating the antifungal activity of novel material.   CONCLUSION PANI/Pull composite was synthetized by MW assisted method under constant irradiation power and temperature. FTIR spectra confirmed the presence of both components, pure PANI and pullulan, in the PANI/Pull composite. Qualitative antimicrobial test showed that PANI/Pull has a high antifungal effect against C. albicans. Even though obtained results from FTIR and antimicrobial evaluation are promising, especially for potential biomedical application, further investigations are required.  Acknowledgement  This work was partially supported by Ministry of Education, Science and Technological Development of Republic of Serbia (Grant No: 451-03-9/2021-14/200146, 451-03-9/2021-14/200168, 451-03-9/2021-14/200026)  REFERENCES [1]  J. S. Karthika, B. Vishalakshi and J. Naik, Int. J. Biol. Macromol., 2016, 82, 61-67. [2] M. Gizdavic-Nikolaidis, G Bowmaker and Z Zujovic in: The synthesis, physical properties, bioactivity and potential applications of polyanilines, Cambridge Scholar Publishing, Newcastle upon Tyne, UK 2018. [3] J. Robertson, M. Gizdavic-Nikolaidis, M. K. Nieuwoudt and S. Swift, PeerJ, 2018, e5135.  [4] V. D. Prajapati, G. K. Jani and S. M. Khanda, Carbohydr. Polym., 2013, 95(1), 540-549. PHYSICAL CHEMISTRY 2021        H-21-P  448  [5] M. B. Coltelli, S. Danti, K. De Clerk, A. Layyeri and P. Morganti, J. Funct. Biomater., 2020, 11(1), 20. [6] M. Goodfellow and D. Minnikin (Eds.), Chemical methods in bacterial systematics, Academic Press, London, UK 1985. [7] K. I. Shingel, Carbohydr. Res., 2002, 337(16),1445-1451.  732  CIP - Каталогизација у публикацији Народна библиотека Србије, Београд  544(082) 66.017/.018(082) 502/504(082) 343.98(082)  INTERNATIONAL Conference on Fundamental and Applied Aspects of Physical Chemistry  (15; 2021; Beograd)     Physical Chemistry 2021: proceedings: the Conference is dedicated to the 30th Anniversary of the founding of the Society of Physical Chemists of Serbia and 100th Anniversary of Bray-Liebhafsky reaction. Vol. 2 / 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 20-24, 2021, Belgrade, Serbia; [organized by The Society of Physical Chemists of Serbia in co-operation with Institute of Catalysis Bulgarian Academy of Sciences ... [et al.]]; [editors Željko Čupić and Slobodan Anić]. - Belgrade: Society of Physical Chemists of Serbia, 2021 (Belgrade: Jovan). - VI str., str. 347-732: ilustr.; 30 cm  Tiraž 200. - Bibliografija uz svaki rad. - Registar.  ISBN 978-86-82475-39-2 ISBN 978-86-82475-40-8 (niz)  а) Физичка хемија -- Зборници б) Наука о материјалима -- Зборници в) Животна средина -- Зборници г) Форензика -- Зборници  COBISS.SR-ID 53325065 

Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite

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Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite

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