The African elephant (Loxodonta africana) is listed as vulnerable, with wild populations
threatened by habitat loss and poaching. Clinical pathology is used to detect and
monitor disease and injury, however existing reference interval (RI) studies for this species
have been performed with outdated analytical methods, small sample sizes or using
only managed animals. The aim of this study was to generate hematology and clinical
chemistry RIs, using samples from the free-ranging elephant population in the Kruger
National Park, South Africa. Hematology RIs were derived from EDTA whole blood
samples automatically analyzed (n = 23); manual PCV measured from 48 samples; and
differential cell count results (n = 51) were included. Clinical chemistry RIs were generated
from the results of automated analyzers on stored serum samples (n = 50). Reference
intervals were generated according to American Society for Veterinary Clinical Pathology
guidelines with a strict exclusion of outliers. Hematology RIs were: PCV 34–49%, RBC
2.80–3.96 × 1012/L, HGB 116–163 g/L, MCV 112–134 fL, MCH 35.5–45.2 pg, MCHC
314–364 g/L, PLT 182–386 × 109/L, WBC 7.5–15.2 × 109/L, segmented heterophils
1.5–4.0 × 109/L, band heterophils 0.0–0.2 × 109/L, total monocytes 3.6–7.6 × 109/L
(means for “regular” were 35.2%, bilobed 8.6%, round 3.9% of total leukocytes),
lymphocytes 1.1–5.5 × 109/L, eosinophils 0.0–0.9 × 109/L, basophils 0.0–0.1 × 109/L.
Clinical chemistry RIs were: albumin 41–55 g/L, ALP 30–122 U/L, AST 9–34 U/L, calcium
2.56–3.02 mmol/L, CK 85–322 U/L, GGT 7–16 U/L, globulin 30–59 g/L, magnesium
1.15–1.70 mmol/L, phosphorus 1.28–2.31 mmol/L, total protein 77–109 g/L, urea
1.2–4.6 mmol/L. Reference intervals were narrower than those reported in other studies.
These RI will be helpful in the future management of injured or diseased elephants in
national parks and zoological settings.The University of Pretoria (UP), the African Wildlife Health and Management Research Theme Fund of the Faculty of Veterinary Science UP, dnata4good-UP’s Wild over Wildlife (WoW) program, the South African government through the South African Medical Research Council and the National Research Foundation South African Research Chair Initiative.https://www.frontiersin.org/journals/veterinary-science#am2022Centre for Veterinary Wildlife StudiesCompanion Animal Clinical StudiesProduction Animal Studie
