The aim of this study was to investigate the occurrence and diversity of pathogenic bacteria in the environment at a livestock farm in Limpopo Province. Environmental samples were collected from three sampling locations: cattle camp (CC), sheep camp (SHC), and goat camp (GC). Samples were processed and analysed for total bacterial counts in the Biotechnology Laboratory, University of Limpopo, South Africa. Identifications were done with Matrix Assisted Laser Disorption Ionisation Time of Flight Mass Spectometry (MALDI-TOF MS) using the simplified on plate technique. The colony forming unit formula per gram of environmental sample (CFU/g) was used to determine total bacterial counts. High counts were observed in CC and low counts in SHC for both soil and faecal samples. Bacillus species were the most dominant across the sampling locations for soil samples. Bacillus cereus occurred in 36 samples (33%), however, the frequency of isolation dominated in the CC with (44.4%), GC (22.2%) and SHC (13%). Staphylococcus aureus had the least frequency (2.2%) followed by Listeria monocytogenes (4.4%). In faecal samples, the occurrence and level of contamination with bacterial species varied across sampling locations with Escherichia coli dominating in the CC with 20% frequency, and abscent from SHC and GC.http://www.aloki.huam2020Veterinary Tropical Disease
