Published by the Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
Babesia occultans, the causative agent of a benign form of cattle babesiosis in South Africa, was continuously
cultivated in microaerophilous stationary-phase culture. A modified medium, 199, supplemented
with either 40% (v/v) bovine or 40% (v/v) horse serum, was used. Cultures were initiated in a humidified
atmosphere containing 2% 0₂, 5% C0₂, and 93% N₂. The highest percentage of parasitized erythrocytes
(PPE) reached 4,5% in horse-serum- and 2,4% in bovine-serum-supplemented medium. Parasite
suspensions were cryopreserved and successfully resuscitated.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201