Phyto-assisted synthesis of zinc oxide nanoparticles (ZnO-NPs) has gained importance because of their stable and eco-friendly nature with significant biological properties compared to chemically synthesized NPs. In the present study biofabrication of ZnO-NPs were carried out using aqueous leaf extract of Sida rhombifolia Linn. The biofabricated ZnO-NPs showed an absorption peak at 307 nm and bandgap energy of 3.51 eV with an average size of similar to 30 nm. The XRD analysis revealed stiff narrow peaks confirming the particles were of no impurities, which were in agreement with EDS analysis. The biofabricated ZnO-NPs exhibited significant antibacterial activity with a MIC of 0.25 mg mL(-1) against E. coli, while it was 0.5 mg mL(-1) against B. subtilis and S. typhi. The live and dead cell analysis of the nanoparticles confirmed that the antibacterial activity was due to damage in the cell walls of the test pathogens. Further, the nanoparticles also offered significant antioxidant and genotoxic properties with an IC50 of 974.5 mu g mL(-1) and 548.4 mu g mL(-1), respectively
