Approximately 156 ornamental species are cultivated via tissue culture in different commercial laboratories worldwide. In vitro plant culture is one of the key tools in plant biotechnology that utilizes the totipotency of plant cells. Pink dianthus (Dianthus chinensis x barbatus) is an important ornamental plant which are grown on balconies, in flower gardens, parks and other during summer period.
In this research the influence of different concentrations and combinations of growth regulators on meristem buds and cotyledons from pink dianthus as starting explants was studied: A - MS + 2 mg/l BA + 0,1 mg/l IAA + 0,1 mg/l GA3; B - MS + 2 mg/l BA + 0,1 mg/l NAA; С - MS + 2mg/l BA and D - MS + 5 mg/l BA + 5 mg/l NAA. The meristem buds cultivated on MS medium supplemented with certain growth regulators developed merely shoots. All combinations of growth regulators under this study resulted in development of roots from shoots in pink dianthus. The newly regenerants were acclimatized with their transfer to sterile mix of perlite and peat (1:1) in high moisture conditions
