Bois noir (BN) has been present in Macedonia for a long time, but its first scientific report dating back to 2003 (Šeruga et al. 2003). BN is caused by phytoplasmas from stolbur group (16SrXII-A). This group of phytoplasma infects a wide range of wild and cultivated plants in Europe. Three phytoplasma isolates associated with BN were identified in grapevine in the world: VKI VKII and VKIII (Langer & Maixner, 2004).
In this study, the spreading of the phytoplasma isolates associated with Bois noir (BN) was investigated on grapevine in the biggest vineyards in Macedonia (Central Vardar region), in the season 2006/07. Used of molecular methods show the presence of Bois noir phytoplasma, type II, VKII, spreader in all investigated regions
