Brzychczy-Włoch, Monika

Drzewiecki, Artur

Fyderek, Krzysztof

Gosiewski, Tomasz

Heczko, Piotr B.

Jedynak-Wąsowicz, Urszula

Kochan, Piotr

Kowalska-Duplaga, Kinga

Pieczarkowski, Stanisław

Strus, Magdalena

Sładek, Małgorzata

Wędrychowicz, Andrzej

Jagiellonian Univeristy Repository

Bifidobacteria numbers in colon of children withinflammatory bowel disease (IBD)Gosiewski T1, Kochan P1, Struś M1, Brzychczy-Wloch M1, Drzewiecki A1, Kowalska-Duplaga K2, Wedrychowicz A2, Jedynak-Wasowicz U2, Sladek M2, Pieczarkowski S2, Fyderek K2, Heczko P.B11 Chair of Microbiology, Jagiellonian University Medical College 2 Clinic of Paediatrics, Gastroenterology and Nutrition, Jagiellonian University Medical CollegeAims of the study:Estimation of changes in the total bacterial flora from the Gl tract of children with primary diagnosis of IBD in comparison to the control group.Materials:Number of subjects in the study: n=64, children, 1-18 years-old hospitalized in the Clinic of Paediatrics, Gastroenterology and Nutrition, Jagiellonian University Medical College Study group: (n=37) IBD patients with primary diagnosis of:1. CD: n=222. UC: n= 123. IC: n= 3Control group: (n=27) patients with functional Gl tract disturbances.Methods:Phenotypic methods -  quantitative microbiological cultures of stool and tissue samples* Culture media: blood agar; McConkey’s agar; Enterococcosel agar; MRS agar; BL agar; Wilkins Chalgren-supl.Agar; Schaedler'sagarMolecular methods* Quantitative FISH from stool samples:Probes: EUB338, STRP, ECOLI, Lab158, Bif 164, BAC303, Erec428* Estimation of the mucus thickness produced by the goblet cellsFluorescently labeled MAA lectin, showing affinity to human mucus polysaccharides.Conclusions:* The bacterial populations in the intestinal lumen in children with IBD, were more numerous than these in children without IBD.* In children with IBD, there were, in comparison to controls, increased numbers of bacteria of Enterobacteriaceae family, including E.coli and also those of Streptococcus genus, but lower numbers of anaerobic bacteria and especially these of Bifidobacterium genus.* The inflammatory process taking place in the intestines of children with IBD, caused a thinning of the mucus layer lining the intestinal wall.This research was supported by the Ministry of Science and Higher Education; grant number 3P05E09125 and 0892/P01/2006/31Results:Content of bacteria in 1 g of faecal matter in the 1st fraction of stool□ Streptococcus■ Lactobacillus□ Enterococcus□ Enterobacteriaceae■ Bifidobacterium□ BacteroidesX2=6,4491*108,p <0,0001 Comparison of the average (%) bacterial content in 1 g of faecal matter in the 1st fraction of stool collected from patients with IBD and from the control group.Content of bacteria in 1 g of faecal matter in the 2nd fraction of stool□ Streptococcus■ Lactobacillus□ Enterococcus□ Enterobacteriaceae■ Bifidobacterium□ Bacteroides%2=6,4491*108, p <0,0001 Comparison of the average (%) bacterial content in 1 g of faecal matter in the Ilnd fraction of stool collected from patients with IBD and from the control group.Content of bacteria in 1 g of faecal matter in the 3rd fraction of stool□ Streptococcus■ Lactobacillus□ Enterococcus□ Enterobacteriaceae■ Bifidobacterium□ Bacteroides%2=6,4491*108, p <0,0001 Comparison of the average (%) bacterial content in 1 g of faecal matter in the Illrd fraction of stool collected from patients with IBD and from the control group.Examples of FISH photographs - combination of Bifl64+EUB probes. Magnification lOOOx. (A) -  stool sample from patient with IBD; (B) -  stool sample from patient without IBD.Thickness of intestinal mucus layer in IBD childrenPhotos showing the thickness of the mucus layer lining the intestinal wall. Magnification 200x. (A) -  intestinal tissue sample from CD patient; (B) -  intestinal tissue sample from UC;(C) -  intestinal tissue sample from control group.

Bifidobacteria numbers in colon of children with inflammatory bowel disease (IBD)

Bifidobacteria numbers in colon of children with inflammatory bowel disease (IBD)

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