Antibacterial, antifungal, hypoglycemic, antihypertensive, analgesic, anti-inflammatory, anti-tumor, anti-viral, urease inhibition, and many more pharmacological effects are related with the 1,2,4-triazole nucleus. Authors created a new series of 4-Amino-5-substituted-3,4-dihydro-2H-[1,2,4]triazole-3-thiols in response to the growing importance of 1,2,4-triazoles as powerful physiologically active agents. Two distinct 2- (5- Mercapto-3-subsituted-1,5-dihydro-[1,2,4]triazol-4-yl) various Schiff bases and -isoindole-1,3-dione(6a-6e) 4-[(4-Dimethylamino-benzylidene)-amino] is a 4-[(4-Dimethylamino-benzylidene)-amino] -5-subsituted-3,4-dihydro-2H-[1,2,4] Different 4-Amino-5-substituted-3,4-dihydro-2H-[1,2,4]triazole-3-thiols were produced and analyzed utilizing spectral techniques such as 1H NMR, 13C NMR, FTIR, and mass spectrometry. Antimicrobial activity against Gram Positive, Gram Negative, and fungal stains was tested on all of these substances. The majority of these compounds have strong antibacterial properties. P. aeruginosa, E. coli, B. subtilis, and B. cerus are all susceptible to compound 6a, 6b, 7c, and 7a. Compounds 7d and 7e were discovered to be effective against P. areuginosa. The typical drugs were Ciprofloxacin and Fluconazole
