https://openworks.mdanderson.org/sumexp22/1029/thumbnail.jp

Aloway, Jace A.

Behringer, Richard R.

Huff, Vicki, PhD

Ruteshouser, Cristy

Terrell, Jasmine L.

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IntroductionEmbryos develop two epithelial ducts, the Wolffian and Müllerian ducts. The Wolffian Duct differentiates into the male-specific sex organs. The Müllerian Duct differentiates into the female-specific sex organs. In the male reproductive tract, the gonads develop into testes which produce testosterone and Anti-Müllerian Hormone (AMH). HypothesisWT1 is a necessary activator of Amhr2 in the Müllerian Duct mesenchyme prior to regression. ResultsReferences1) Klattig, J. et al. Wilms’ Tumor Protein Wt1 Is an Activator of the Anti-Müllerian Hormone Receptor Gene Amhr2. Molecular and Cellular Biology 27, 4355-4364 (2007).MethodsGoal: Block Amhr2 expression in the Müllerian Duct mesenchyme by knocking out Wt1 We used CRE to block WT1 in the Müllerian Duct mesenchyme and prevent expression of Amhr2. Role of Wilms’ Tumor 1 in Sex Development  Jasmine L. Terrell1, Jace A. Aloway2, Cristy Ruteshouser2, Vicki Huff2, Richard R. Behringer21Department of Biology, Howard University; 2Department of Genetics, MD Anderson Cancer CenterFrom the testes, AMH will travel to the Müllerian Duct mesenchyme to signal its receptor, Anti-Müllerian Hormone Type II Receptor (AMHR2). Once AMH & AMHR2 bind, the Müllerian Duct will begin to regress. Ultimately, this will leave the Wolffian Duct which will further develop into the male reproductive system. Mutations in Amhr2 leads to Persistent Müllerian Duct Syndrome (male with a uterus). AcknowledgementsDiana Machado, Malcolm Moses, Bonnie Kircher, Rachel MullenWilms’ Tumor 1 (WT1) is co-expressed with AMHR2 in the Müllerian Duct mesenchyme prior to and during regression.   Red Fluorescent Protein (RFP) is activated from the allele to highlight cells that express the recombined WT1 allele.  ♀Control Uterus♂WT1 CKO♂Control Vas DeferensWt1 RFPΔ/+ ♂ Reproductive Tract Seminal VesicleTestisVas Deferens UterusUterusOvaryOviductsOverall, the male knockout mouse has a uterus. Red Fluorescence in Wt1 RFPΔ/+Female Reproductive SystemUterusVas Deferens

Role of Wilms\u27 Tumor 1 in Sex Development

https://openworks.mdanderson.org/cgi/viewcontent.cgi?article=1029&amp;context=sumexp22

Role of Wilms\u27 Tumor 1 in Sex Development

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