Asins, María J.

Belver, Andres

Espinosa, Jesús

González-Fernández, Paloma

Jaime-Fernández, Emilio

Romero-Aranda, María R.

Traverso, Jose A.

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XV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS Online, November 26-27th 2020Name & Surname of the presenting author: Andrés BelverE-mail: andres.belver@eez.csic.esSession: Abiotic StressCommunication (Oral or Poster): PosterSALT TOLERANCE EFFECTS OF SOLANUM CHEESMANIAEHKT1;1 AND/OR HKT1;2 FUNCTION LOSS IN A RECIPROCALGRAFTING TOMATO EXPERIMENT*María Remedios Romero-Aranda1, Jesús Espinosa2, Paloma González-Fernández2,Emilio Jaime-Fernández1, José Ángel Traverso3, María José Asins4, Andrés Belver2 1IMSH "La Mayora", UMA/CSIC, Málaga;  2Estación Experimental del Zaidín, CSIC,Granada; 3Faculty of Sciences, University of Granada;.4 IVIA, Moncada, Valencia Corresponding author: Andrés Belver (andres.belver@eez.csic.es)HKT1-like Na+ transporters play an important role in Na+ and K+ homeostasis and salttolerance  by  removing  Na+ from the  xylem controlling  its  accumulation  in  shoots(Asins et al.  2013).  In previous studies, we used recombinant inbred lines (RILs)derived  from  Solanum  lycopersicum cv.  Cerasiform  and  S.  cheesmaniae,  ahalotolerant wild species, combined with RNAi-induced loss-of-function in all HKT1;1and HKT1;2 allelic variants. We showed that HKT1;2 lycopersicum and cheesmaniaealleles are functionally responsible for the major tomato QTL lkc7.1 related to Na+/K+homeostasis in the aerial part of the plant under saline conditions (Asins et al. 2013;Jaime-Pérez et al. 2017). In the present study, we analyse the physiological impactof function loss in  cheesmaniae alleles at the  HKT1;1 and HKT1;2 loci in the rootsand aerial parts of the tomato plant in order to determine the relative contributions ofeach locus in the different tissues to plant Na+/K+ homeostasis and subsequently totomato salt tolerance. We generated different reciprocal rootstock/scion combinationswith non-silenced, single RNAi-silenced lines for  ScHKT1;1 and  ScHKT1;2, as wellas doubly silenced lines,  at  both loci  from a near  isogenic line (NIL14),  which ishomozygous  for  the  S.  cheesmaniae  haplotype  in  a  genomic  region  (31.1  Mb)containing  both  HKT1 loci  (Jaime-Pérez et  al.  2017).  Grafted  combinations werecultivated with a Hoagland nutrient solution supplemented with 0 and 70 mM of NaClunder natural greenhouse conditions during the fall-spring season following the usualcultural practices of commercial greenhouse tomato production (Romero-Aranda etal. 2020). Our results show that salt treatment reduced vegetative growth and alteredthe  Na+/K+ ratio  in  leaves  and  flowers,  negatively  affecting  fruit  production,particularly  in  graft  combinations  containing  ScHKT1;2-silenced  and  double-SlHKT1;2/ScHKT1;1 silenced lines,  when used for  both  rootstock  and scion.  Weconcluded that the impact of the removal of Na+  from the xylem by ScHKT1;2 in theaerial part of the plant can be even greater than that on Na+ homeostasis at the rootlevel in tomato under saline conditions. This suggests that ScHKT1;2 may also play arole in Na+ phloem loading and consequently in determining Na+ recirculation towardsthe root zone, as well as the salt content of sink tissues such as developing leaves,flowers and  fruits.Asins et al.  (2013). Plant Cell  and Environment 36: 1171-91. Jaime-Pérez et al.  (2017) Plant CellEnvironm 40, 658–671. Romero-Aranda et al. (2020) Plant Physiol Biochem 154, 341-352.*ERDF-cofinanced grants from AEI, AGL2017-82452-C2-1R (AB) and AGL2017-82452-C2-2R (MJA).Graphical abstract (Optional)GuidelinesThe graphical abstract is one single panel image that is designed to give readers an immediate understanding of the take home message of the work. It should take no more than half page of space.El contenido del abstract gráfico es propiedad de los autores del mismo. Los organizadores no serán responsables del uso indebido de imágenes ni de la infracción de la ley de propiedad intelectual.

Salt tolerance effects of Solanum cheesmaniae HKT1; 1 and/or HKT1; 2 function loss in a reciprocal grafting tomato experiment

http://redivia.gva.es/bitstream/20.500.11939/7463/1/2020_Romero-Aranda_Salt.pdf

Salt tolerance effects of Solanum cheesmaniae HKT1; 1 and/or HKT1; 2 function loss in a reciprocal grafting tomato experiment

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