Meise, Philipp

Ordon, Frank

Schum, Annegret

Seddig, Sylvia

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2014 Meise et al.     Morphological, physiological and proteomic responses of potato cultivars to nitrogen  deficiency in an in vitro system  Philipp Meise1, Annegret Schum1, Sylvia Seddig1, Frank Ordon1 1 Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance, Groß Lüsewitz  Email of corresponding author: philipp.meise@jki.bund.de  Nitrogen is an essential nutrient which directly influences crop yield and quali-ty. Potatoes require high levels of nitro-gen up to 200 kg per hectare, especially in early developmental stages for cano-py development. The above-ground biomass is generally related to the  photosynthetic capacity and therefore contributes essentially to tuber yields. Nitrogen fertilizers have been used in-tensively with negative side effects on the environment, e.g. nitrate leaching to the groundwater. This is a special problem in potato production due to the shallow root system of potato and the practice of total nitrogen input in a single treatment at the beginning of growing. Identification of traits contrib-uting to improved nitrogen uptake and utilization may help in breeding of po-tato cultivars with optimized nitrogen efficiency. Previous studies on in vitro grown table potatoes indicated that responses to nitrogen deficiency varied considerably in a set of table potato cultivars. Espe-cially differences in nitrogen uptake capacity and root development were observed. This suggested cultivar-depend differences in response to ni-trogen deficiency and nitrogen use effi-ciency. In the present study the devel-oped method was used to screen a set of starch potato cultivars for their re-sponse to nitrogen deficiency under highly controlled in vitro conditions. Liquid MS media with four nitrogen levels (60, 30, 15 and 7.5 mMol/l) were used and plant development and nitro-gen uptake evaluated after 7, 11, 14 and 18 days of incubation. Here we present results obtained on two table cultivars (Lambada and To-pas) and seven starch cultivars. Lambada produces high biomass under high nitrogen availability and reacts with a strong decrease under nitrogen deficiency. In contrast Topas produces less biomass under high nitrogen avail-ability, but does not react with a strong decrease under limited conditions. The nitrogen uptake and root development on Lambada is faster than on Topas. We use these differently reacting cultivars to classify the starch cultivars for their response to nitrogen deficiency. To achieve this we determine the biomass production of roots and shoots (fresh and dry matter), the chlorophyll- and crude protein content. Furthermore, differences in the nitrogen uptake dur-ing the cultivation period will be demonstrated. In addition, the in vitro system is used to study early proteomic changes in the shoot proteome on the cultivars Topas and Lambada. Using 2D IEF/SDS PAGE method to separate and MALDI-TOF MS to indentify the proteins. First results obtained revealed more differently regulated proteins in Topas than in Lambada. 38 Berichte aus dem Julius Kühn-Institut 177

Morphological, physiological and proteomic responses of potato cultivars to nitrogen deficiency in an in vitro system

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Morphological, physiological and proteomic responses of potato cultivars to nitrogen deficiency in an in vitro system

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