Bziuk, Nina

Lauterbach, Desirée

Schikora, Adam

Smalla, Kornelia

Sørensen, Søren J.

English

JKI Open Journal Systems (Julius Kühn-Institut)

11th Young Scientists Meeting 2018, Braunschweig, Germany, November 14-16 Bziuk et al. Enhanced plant resistance towards phyto-pathogenic fungi depends on the rhizosphere microbial community composition Nina Bziuk1, Desirée Lauterbach1, Søren J. Sørensen2, Adam Schikora1 and Kornelia Smalla1 1 Julius Kühn Institute, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig 2 University of Copenhagen, Department of Biology, Section of Microbiology, Copenhagen E-mail of corresponding author: nina.bziuk@julius-kuehn.deThe plant’s resistance towards phyto-pathogenic fungi is assumed to be en-hanced by interactions with its rhizomi-crobiota. It is known that beneficial bac-teria in the rhizosphere can trigger a faster and stronger immune response of the plant towards pathogens or abiotic stresses, which can also be called prim-ing. Furthermore, the prokaryotic com-munity compositions of agricultural soils differ depending on the applied manage-ment practice. We hypothesize that pro-karyotic communities from different agri-cultural legacy might also influence the ability of the rhizomicrobiota of barley cultivar ‘Golden Promise’ to enhance the resistance against powdery mildew Blu-meria graminis f. sp. hordei. Therefore, an experimental approach was devel-oped suitable to test the priming ability of the rhizomicrobiota under greenhouse conditions. Detached barley rhizomicro-biota from plants grown in field soil was inoculated to a substrate/sand mixture, which was planted with barley seedlings. Control plants were treated in the same way but with saline solution. At growth stage 13, barley plants were infected with B. graminis, control plants were leftuntreated. The prokaryotic community composition was analyzed by sequencing of 16S rRNA genes amplified from total community DNA directly extracted from rhizosphere soils sampled nine days after infection. The priming efficiency was ex-amined by a detached leaf assay and ex-pression pattern of defense-related genes analyzed by qPCR. Although the re-sistance against B. graminis was not found to be improved, plants treated with the rhizosphere microbiota showed a stronger defense response after the fungal infection. Furthermore, an influ-ence of the rhizosphere inoculant, as well as the presence of the fungal pathogen on the prokaryotic rhizosphere commu-nity with several differentially abundant taxa was observed. Our results suggest a stronger priming ability of the rhizomicrobiota from field soil compared to the substrate commu-nity. The developed approach proved to be suitable for testing the abilities of pro-karyotic communities originating from soils with different agricultural history to enhance the resistance of barley towards fungal phytopathogens.  50 Berichte aus dem Julius Kühn-Institut 200

Enhanced plant resistance towards phytopathogenic fungi depends on the rhizosphere microbial community composition

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Enhanced plant resistance towards phytopathogenic fungi depends on the rhizosphere microbial community composition

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