Eibach, Rudolf

Schwander, Florian

Töpfer, Reinhard

Zyprian, Eva

English

JKI Open Journal Systems (Julius Kühn-Institut)

Nachwuchswissenschaftlerforum / Young Scientists Meeting 2011  Florian Schwander et al.  Berichte aus dem Julius Kühn-Institut 162, 2011  Localisation and fine-mapping of the downy mil-dew resistance locus Rpv10 in grapevine  Florian Schwander, Rudolf Eibach, Eva Zyprian, Reinhard Töpfer Julius Kühn-Institut, Institute for Grapevine Breeding florian.schwander@jki.bund.de The pathogen Plasmopara viticola causing downy mildew is an oomycete native to North America. It was distributed through-out almost all wine growing regions around the world. Interestingly several accessions of the Asian wild grape Vitis amurensis possess resistance to P. viticola. Introgres-sion of this resistance source into the gene pool of the European high quality grape-vine V. vinifera resulted in new cultivars, such as ‘Solaris’, resistant to downy mil-dew. QTL analysis of a cross population derived from a cross between the breeding strain Gf.Ga-52-42 and the cultivar ‘Solaris’ revealed the resistance locus Rpv10 on chromosome 09. A subsequent fine map-ping resulted in confidence intervals of less than one cM corresponding to 79 kb be-tween the flanking markers on the grape-vine reference genome. Four potential re-sistance candidate genes were identified in this section of the sequence. SSR markers flanking the locus can be used in marker assisted breeding (MAS) for screening ge-netic resources and breeding material. A set of 94 V. amurensis descendants was screened and 22 genotypes carrying the Rpv10 locus were identified. These geno-types are potential sources to introduce the Rpv10 locus into the breeding process for pyramiding resistance loci by combining different resistance donors. This is ex-pected to result in plants which possess high and durable resistance properties. MAS offers the possibility to select these promising genotypes. By taking advantage of newly developed markers for Rpv10 the locus can be used immediately in resis-tance breeding to achieve high level of downy mildew resistance by pyramiding with further resistance loci.     23

Localisation and fine-mapping of the downy mildew resistance locus Rpv10 in grapevine

https://ojs.openagrar.de/index.php/BerichteJKI/article/download/1644/1977

Localisation and fine-mapping of the downy mildew resistance locus Rpv10 in grapevine

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