The aim of the research was to identify Allium ascalonicum land races with a good yield due to their morphological traits, with good adaptation capacity, with different genetic background, as a first step in identification of a suitable source for the production of secondary metabolites. Therefore 16 shallot landraces collected from different areas of Timis county were evaluated from phenotypic and molecular point of view. Thus the height, diameter, weight and bulb shape index were determined. For genetic fingerprint 8 ISSR (Inter Simple Sequence Repeats) markers were used. The extracted DNA was amplified with the specific primers, the fragments were separated by agarose gel electrophoresis and analyzed with the VisionWorks®LS, (UVP, England) software. 178 amplified fragments were registered, with an average of 22.25/primer, of which 174 were polymorphic (97.75% polymorphism). The matrix of similarity and the dendrogram were established based on UPGMA (Unweighted Pair Group Method with Arithmetic Mean) analysis. A considerable genetic diversity between landraces with different ecologic origin was observed, indicating that they have different genetic mechanisms for the yield traits and adaptation to the specific environmental conditions of the area. All the obtained results allowed the identification of local land races with a high production capacity which had different genetic background (Dolat 126a, Sanmartinu S. and Rudna 101.) Therefore the posibility to recognize genotypes with distinct secondary metabolites content it was increased
