The young leaves and ovules of Plukenetia volubilis were cultured in MS medium under 5 different treatments of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) incubated in different conditions, either 24 hours light or 24 hours dark condition to develop an in vitro micropropagation through callus induction. Despite a series of contamination after surface sterilization, the young leaves explants deemed promising in developing callus in media supplemented with both plant growth regulators of 0.1 mg L-1 2,4-D + 0.05 mg L-1 BAP and 1.0 mg L-1 2,4-D + 0.05 mg L-1 BAP. Interestingly, it is the similar concentration that yielded callus from ovule. All treatments developed callus from ovule, in which the best callus was induced from MS media supplemented with 0.1 mg L-1 2,4- D + 0.05 mg L-1 BAP in both light and dark conditions compared to the single use of 2,4-D. This is the first report attempted on using ovules of sacha inchi as explant
