poster C149, Proceedings Annual Biomedical Research Conference for Minority Students (ABRCMS 2011), November 9 - 12, 2011, America’s Center, St. Louis, MO, US

A. N. Blood

BOSELLI, EMANUELE

J. M. D. Dimandja

English

IRIS UniversitÃ  Politecnica delle Marche

Print this Page    Presentation AbstractSession: Poster Session 3 (C)Friday, Nov 11, 2011, 11:00 AM -12:15 PMPresentation Title:C149 - Profiling of Berries by Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry (GCxGC/TOF-MS)Location: Exhibit Hall 4 , Section: ChemistryScientific Discipline:+ 4. Chemistry -> a. Analytical ChemistryAuthor(s): Allisha Blood1; Jean-Marie D.. Dimandja, Ph.D.1; and Emanuele Boselli, Ph.D.2 Spelman College, Atlanta, GA1 and Universita Politecnica delle Marche, Ancona, Italy2Abstract: Hypothesis/statement of problem - Comprehensive bidimensional gas chromatography coupled with TOF-MS is a promising analytical technique for the challenging task of fingerprint natural chemical markers of different kinds of berries. This is important information both for food quality control and for the identification of peculiar components of small fruits, such as natural organic acids, amino acids and antioxidants. Experimental methods - The juice (50 mL) was obtained upon mixing and centrifuging of about 200 g of fresh or frozen berries and was acidified with H2SO4 to pH 1. A solution of heptadecanoic acid was added as an internal standard. A fraction of the dichloromethane extract of the acidified juice was dried under gentle nitrogen flow, and silylated with BSTFA containing 1% TMCS in pyridine. The TMS extract was then injected in a Pegasus 4D comprehensive two-dimensional gas chromatograph with time-of-flight detection (GCxGC/TOF MS). The column combination was as follows: the 1st column was a (5% diphenyl) Rxi-5ms 30m x 250 μm i.d. with a 0.25 μm film thickness; the 2nd column was a (50% diphenyl) Rxi-17ms 0.79m x 100 μm i.d. with a 0.1 μm f.t. The inlet temperature was 250°C; the temperature program Page 1 of 2Abstract Print View22/11/2011http://www.abstractsonline.com/Plan/AbstractPrintView.aspx?mID=2809&sKey=374...  Annual Biomedical Research Conference for Minority Students  TECHNICAL SUPPORT: 217-398-1792 (Monday through Friday 9:00 am-5:00 pm Central Standard Time) or OASIS Helpdesk   for the oven started from 40 °C kept for 2 min and raised to 280 °C at 20 °C/min. The final isothermal phase was maintained for 16.5 min. The temperature of the transfer line was 290 °C. The modulation time was 4 sec. Results - The juice of different kinds of fresh and frozen berries was characterized by means of GCxGC/TOF-MS. The main components found in the tridimensional chromatographic trace (1st column and 2nd column retention times plus abundance) were organic acids, aminoacids and phenolic compounds identified through their fragmentation pattern, retention times and by injecting standard solutions. The main advantages of the GCxGC/TOF MS analysis when compared to one dimension GC/MS are higher separation efficiency and the reduction of interferences due the column bleeding, which allows for the detection of a significant number of trace analytes that would otherwise be missed. Conclusion - It is possible to apply comprehensive GCxGC/TOF-MS not only for the determination of the aroma profiles of fruits and fruit products (as reported in previous literature) but also for the characterization of the non- volatile fractions of the juice which contains low molecular weight components potentially useful for health/pharmaceutical/nutraceutical applications and for food quality assurance.Page 2 of 2Abstract Print View22/11/2011http://www.abstractsonline.com/Plan/AbstractPrintView.aspx?mID=2809&sKey=374...

Profiling of berries by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GCxGC/TOF-MS)

https://iris.univpm.it/bitstream/11566/63384/1/StLouis2011.pdf

Profiling of berries by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GCxGC/TOF-MS)

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