Burkholderia plantani is a plant pathogen causing rice seedling blight. It produces tropolone, the disease causing substance. To elucidate and understand molecular mechanisms of the pathogenesis, we analyzed the genome of B. plantarii. In this study, we focused on two component system, in prokaryotic signal transduction. This system is basically composed of a histidine kinase (HK, sensor) residing in the inner membrane and a cognate response regulator (RR) in the cytoplasm. Here, BasS, a histidine kinase (HK, sensor) and BasR, a response regulator (RR) in this strain, were structurally analysed, showing these are tandemely ordered. Phylogenetic analysis of these prodcuts were also analysed. Construction of plasmids for gene disruption of these genes, basS and basR was also carried out, in terms of future study approach in reverse genetics. Obtaining mutants by transformation will be useful for understanding the relationships between pathogenesis and function of these genes
