Additional file 4 of Metabolomics detects clinically silent neuroinflammatory lesions earlier than neurofilament-light chain in a focal multiple sclerosis animal model

Abstract

Additional file 4: Fig. S4 Comparison of clean vs. blood-contaminated CSF NMR spectra. (A) 1D-NOESY-presat spectra (chemical shift shown: 0.60 ppm – 4.20 ppm) demonstrating clean CSF (in blue) and blood-contaminated CSF (in red). This revealed aberrant NMR resonances contributed by lipoproteins and macromolecules (e.g. albumin) in blood, resulting in a broad signal with an elevated baseline (boxed region, chemical shift shown: 0.70 ppm – 1.40 ppm). (B) Zoom-in view of this boxed region showed additional resonances arising from the mobile methyl (CH3) and methylene (CH2) groups within lipoproteins, as well as from the branched chain amino acids (i.e. isoleucine, leucine, and valine). NMR: nuclear magnetic resonance; NOESY: nuclear overhauser effect spectroscopy; ppm: parts per millio