Today, clothing systems for cleanrooms and associated controlled environments such as ultra clean air operating rooms are mainly tested with regard to material properties, such as particle generation, particle filtration, resistance to wear and comfort. The dispersal chamber or body-box has been used for studying the protective efficacy of clothing systems in use. A modified dispersal chamber has been installed at Chalmers University of Technology, Gothenburg. Tests have been performed in the dispersal chamber on two Olefin surgical clothing systems with textile hoods, one system with shoes and the other system with textile knee-length boots over the shoes.The results show clearly that the clothing systems with textile knee-length boots has better protection efficacy than that without knee-length boots