Analysis of TFF3 presence in skin tumours

Abstract

CILJ ISTRAŽIVANJA. Cilj ovoga istraživanja bio je utvrditi postoji li izražaj TFF3 proteina u primarnim tumorima kože, postoje li značajne razlike u izražaju TFF3 proteina u zdravoj koži i tumorski promijenjenoj koži te postoje li značajne razlike između različitih vrsta tumora kože. USTROJ STUDIJE. Uzorci su imunohistokemijski obojeni, histomorfološki analizirani svjetlosnim mikroskopom (kvalitativno) i semikvantitivno je analizirana jačina signala brojanjem TFF3 pozitivnih stanica. MATERIJAL I METODE. Istraživanje je provedeno na sveukupno 53 uzorka, 12 uzoraka zdrave kože, 14 uzoraka bazocelularnog karcinoma, 15 uzoraka planocelularnog karcinoma i 12 uzoraka malignog melanoma. Preparati su imunohistokemijski obrađeni te analizirani pomoću svjetlosnog mikroskopa. Dobiveni podaci obrađeni su u statističkom programu MedCalc Statistical Software version 15.11.4 (MedCalc Software bvba, Ostend, Belgium). REZULTATI. Morfološkom analizom uočen je izražaj TFF3 proteina u primarnim tumorima kože, kao i u epidermisu zdrave kože. Izražaj TFF3 proteina bio je statistički značajno manji kod bazocelularnog i planocelularnog karcinoma kože u odnosu na zdravu kožu, za razliku od tumora dojke gdje postoji povećani izražaj TFF3 proteina. Između različitih tumora kože nije nađena značajna razlika u izražaju TFF3 proteina. ZAKLJUČAK. Postoji izražaj TFF3 proteina u primarnim tumorima kože, ali se po intenzitetu značajno ne razlikuje među pojedinim tipovima tumora. Izražaj TFF3 značajno je manji u bazocelularnom i planocelularnom karcinomu nego u zdravoj koži.OBJECTIVES. The aim of this study was to determine is there any expression of TFF3 protein in primary skin tumours, whether there are significant differences in the expression of TFF3 protein in healthy skin and skin tumours and are there any significant differences between the different types of skin cancer. STUDY DESIGN. The samples were stained using immunohistochemistry, histomorphologically analyzed by light microscopy (qualitative) and the signal strength was semiquantitatively analyzed by counting TFF3 positive cells. MATERIAL AND METHODS. The study was conducted on a total of 53 samples, 12 samples of healthy skin, 14 samples of basal cell carcinoma, 15 samples of squamous cell carcinoma and 12 samples of malignant melanoma. Slides were processed and analyzed by immunohistochemistry using a light microscope. The obtained data were processed using statistical program MedCalc Statistical Software version 15.11.4 (MedCalc Software BVBA, Ostend, Belgium). RESULTS. Expression of TFF3 protein was noticed in primary skin tumours as well as in the epidermis of healthy skin by morphological analysis. The expression of TFF3 protein was significantly lower in basal cell and squamous cell skin cancer compared to healthy skin, unlike breast cancer where there is an increased expression of the TFF3 protein. Among the various types of skin cancer there was not a significant difference in the expression of proteins TFF3. CONCLUSION. TFF3 protein expression was present in primary tumours of the skin but no significant differentes were cound in staining intensity between different tumour types. TFF3 expression was significantly lower in the basal cell and squamous cell carcinoma than in healthy skin