Allergic airway inflammation induces upregulation of the expression of IL-23R by macrophages and not in CD3 + T cells and CD11c+F4/80- dendritic cells of the lung
Interleukin 23 and the interleukin 23 receptor (IL-23-IL23R) are described as the major enhancing factors for Interleukin
17 (IL-17) in allergic airway infammation. IL-17 is considered to induce neutrophilic infammation in the lung, which is
often observed in severe, steroid-resistant asthma-phenotypes. For that reason, understanding of IL-23 and IL-17 axis is
very important for future therapy strategies, targeting neutrophil pathway of bronchial asthma.
This study aimed to investigate the distribution and expression of IL-23R under physiological and infammatory conditions.
Therefore, a house dust mite (HDM) model of allergic airway infammation was performed by treating mice with HDM
intranasally. Immunofuorescence staining with panel of antibodies was performed in lung tissues to examine the macrophage,
dendritic cell, and T cell subpopulations. The allergic airway infammation was quantifed by histopathological analysis,
ELISA measurements, and airway function.
HDM-treated mice exhibited a signifcant allergic airway infammation including higher amounts of NE+ cells in lung
parenchyma. We found only a small amount of IL-23R positives, out of total CD3+T cells, and no upregulation in HDMtreated animals. In contrast, the populations of F4/80+ macrophages and CD11c+F4/80− dendritic cells (DCs) with IL-23R
expression were found to be higher. But HDM treatment leads to a signifcant increase of IL-23R+ macrophages, only. IL23R was expressed by every examined macrophage subpopulation, whereas only Mϕ1 and hybrids between Mϕ1 and Mϕ2
phenotype and not Mϕ2 were found to upregulate IL-23R. Co-localization of IL-23R and IL-17 was only observed in F4/80+
macrophages, suggesting F4/80+ macrophages express IL-23R along with IL-17 in lung tissue.
The study revealed that macrophages involving the IL-23 and IL-17 pathway may provide a potential interesting therapeutic
target in neutrophilic bronchial asthma
