In the course of screening new streptomycete strains, the strain Streptomyces sp. Cl 58-27
caught our attention due to its interesting secondary metabolite production profile. Here, we report
the isolation and characterization of an ansamycin natural product that belongs structurally to the
already known kendomycins. The structure of the new kendomycin E was elucidated using NMR
spectroscopy, and the corresponding biosynthetic gene cluster was identified by sequencing the
genome of Streptomyces sp. Cl 58-27 and conducting a detailed analysis of secondary metabolism
gene clusters using bioinformatic tools