Minnick, Michael F.

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University of Montana

University of Montana ScholarWorks at University of Montana Biological Sciences Faculty Publications Biological Sciences 2-25-1993 Nucleotide Sequence of the 5S Ribosomal RNA Gene of Bartonella bacilliformis Michael F. Minnick University of Montana - Missoula, mike.minnick@mso.umt.edu Follow this and additional works at: https://scholarworks.umt.edu/biosci_pubs  Part of the Biology Commons Let us know how access to this document benefits you. Recommended Citation Minnick, Michael F., "Nucleotide Sequence of the 5S Ribosomal RNA Gene of Bartonella bacilliformis" (1993). Biological Sciences Faculty Publications. 190. https://scholarworks.umt.edu/biosci_pubs/190 This Article is brought to you for free and open access by the Biological Sciences at ScholarWorks at University of Montana. It has been accepted for inclusion in Biological Sciences Faculty Publications by an authorized administrator of ScholarWorks at University of Montana. For more information, please contact scholarworks@mso.umt.edu. 1036 Nucleic Acids Reseatch, 1993, Vol. 21, No. 4Nucleotide sequence of the 5S ribosomal RNA gene ofBartonella bacilliformisMichael F.MinnickDivision of Biological Sciences, The University of Montana, Missoula, MT 59812. USAReceived January 11, 1993: Accepted January 14 1993Eubacterial rRNA genes are usually organized in opeironscontaining the 16S, 23S and 5S rRNA genes, in that order (I).A cluster of structural rRNA genes was discovered duringnucleotide sequencing of a cloned 3.6-kb BamiiHI fragment ofDNA from B. bacillifornis, the agent of Oroya fever in humans.The 5S rRNA gene is 119 bp in length and is located 107 bases3' to the 23S rRNA gene of the bacterium (Figure IA). The genecontains the highly conserved eubacterial 5S rRNA sequence G-AAC (Figure IB), which is believed to interact with tRNA'sduring translation (2). The 5S gene is immediately preceded andfollowed by a 12-base inverted repeat flanked by adenine-richsequences (Figure IB). Previous homology searches using the1 6S rRNA sequence of B. bacilliformnis indicated that thebacterium was closely related to Rochalirnaea quintanti. Brucellaiabortus, and Agrobacteriiumni tumniefacien.s with 91.7 %. 91.37(%.and 88.8% identity, respectively (3). In keeping with the highdegree of 16S rRNA homology (3). searches with the 5S rRNAgene from this study show 84% sequence identity to the 5S geneof A. tumefacienis. The 5S sequence homology also suggests thatB.bacilliformnis may be closely related to Vibrio cvclosite.s. with91.6% 5S rRNA sequence identity (4). As the 16S rRNAsequence from V. cvclosites and the 5S rRNA sequences ofR. quintana and B. abortus become available, a further homologycomparison will be useful for the proper classification ofB.bacilliformis. The bacterium is presently placed in the orderRickettsiales. However, it is the only motile species in the orderand is one of just two genera, including Rochalirnaea. which canbe cultured axenically (5).ACKNOWLEDGEMENTSThis work was supported by funds from the University ofMontana URCAC and MONTS program (#RII 8912934).Synthetic oligonucleotides were provided by the University ofMontana Molecular Biology FacilitY.GenBank accession no. L085033. Brncr. D .J . ()'Connor.S. P.. HolliN.l ).. WcavcRetK In (dSteiBerwalt.A.G. (1991) J. C/lin. Microbiol. 29. 1299- 1302.4. Nluir.D.G., Hori.H.. Ortliz-Conde.B.A.. ArnilkisM.S. Laind Colwell,R.R.(1989) Nucleic Acids Re,.s 18. 1636.5. RisticAl. and Kreier.J.P. (1984) In: Kric ..N .R. .and HoltJG. (eds). Bcrgcv',.Mailatil of' Sstematic bacteriologxv. 'Nil l aiis andl Wilkins Co.. Baltimilorc.NID. Vol. 1. P 7l7 719.AB1 AAGCTTACTG ATACTAATAG TTCGATTGG? TTGATC3TTC TCATTTCCTC TATTCATTCA61 TGATGAAACA CTCAATTAAT GAATAAAACT AAAACTTAAT AAAAGCACAA AACCAGTTTC121 TCAATTCTAT GCCCTTTGCT GACTTGGTGG TTATGGCGGA ACGCCTGCAC CCGATCCCAT181 CCCGAACTCG GCCGTGAAAC GTTCCAGCGC TGATGGTACT TTGTCTTAAG GCACGGGAGA241 GTAAGTCGCT GCCAGGTCTG CTAAGGGCAT AGAAACATCC TCACAATCTT CAAAAAATTC301 TGTCATTTTA AAAGAAGCCA ATTTAAAAAA GACAAACAAC AAAAAGAAGA AAAACACCCA361 AAATAGGGCC GTATCTTCTT CATGGCGCGG GGTGGAGCAG CCCGGTAGCT CGTCAGGCTCFigure 1. Characterization ot the 5S rRNA gcnc ot B. baicllfi)rmni,s. A. Partialrestriction map of the BamiiHI f'ragment of plMINII showing the positions of the23S (3' end) and 5S rRNA aenes. Abbreviations: B. BaRHI; E. E(oRI: H. HinldIIl:P. PstI: S. So/I. B. Nucleotide sequence of the 5S g-,ene (uniderlined) and its flankinpsequences. The conserved GAAC sequcic is ovrnlincd. Inverted rcpcats ar1cindicaIted bv arrows above the sequence.REFERENCES1. Nornura,M., Morgan,E.A. and Jaskunas,S.R. (1977) Aun. Rev. Genet. 11.297 - 347.2. Noller,H.F. (1984) Ann,uii. Rev. Biochemn. 53. 119- 162.1993 Oxfi)rd Universitv Press

Nucleotide Sequence of the 5S Ribosomal RNA Gene of Bartonella bacilliformis

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Nucleotide Sequence of the 5S Ribosomal RNA Gene of Bartonella bacilliformis

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