Received: March 16th, 2022 ; Accepted: July 20th, 2022 ; Published: September 6th, 2022 ; Correspondence: [email protected] identification of effective specialized DNA markers providing the clear control of
target locus inheritance by the trait of submergence tolerance has been conducted. Among the
studied set of microsatellite markers, two the most informative SSR-markers - RM 7481, PrC3
showed high efficiency in detecting intraspecific polymorphism of rice varieties and lines used
in the work. With the use of these markers the clear genotype marking the obtained hybrid rice
plants by this trait has been conducted and it is has been verified by phenotype evaluation as a
result of laboratory trials. The plant samples carrying the target gene in heterozygous and
homozygous state has been selected. About 400 backcrossed self-pollinated rice lines with
introgressed and pyramided resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, Pi-b to Pyricularia oryzae
Cav. were obtained within the frameworks of program to develop genetic rice sources resistant
to blast. The conducted testing for resistance to blast and the assessment by economically valuable
traits have allowed to select the prospective rice samples. The plant samples of F2 and BC1F1
generations with combination of resistance to blast genes (Pi) and submergence tolerance gene
(Sub1A) in homozygous and heterozygous state that is confirmed be the results of analysis of
their DNA have been obtained. The obtained hybrid plants are being tested in breeding nurseries
for a complex of economically valuable traits. The best plants will be selected and send to State
Variety Testing system. Their involving in rice industry will reduce the use of plant protection
chemicals against diseases and weeds, thereby increasing the ecology status of the rice industry
