Alinaitwe, Lordrick

Bugeza, James

Cook, Elizabeth A.J.

Kankya, C.

Kivali, Velma

Moriyon, I.

Mugizi, Denis

CGSpace

Epidemiology of brucellosis at thehuman-livestock interface in UgandaJames Bugeza1,2, Clovice Kankya2, Denis Mugizi1, Velma Kivali1, Lordrick Alinaitwe1, Ignacio Moriyon3, Elizabeth Cook11ILRI Uganda/Kenya; 2Makerere University, Uganda; 3Universidad de Navarra, SpainIntroductionBrucellosis is a major food borne and occupational zoonosis; and a livestock production constraint in Uganda. Comprehensive knowledge of itsepidemiology is vital for its progressive control and eradication from endemic areas.Aim of the study is to determine: 1. The seroprevalence of anti-Brucella antibodies and exposure factors in both livestock and slaughterhouse workers atthe point of slaughter; 2. The circulating species, their origin and phylogeny and 3. Resistance to first line antibioticsMethodsPicturesConclusions• Cattle and shoats seem to play a major role in the epidemiological cycle of brucellosis compared to swine• All regions are affected• There is occupational exposure to Brucella at the point of slaughter → Occupational safety implicationsLimitations• Tissue deterioration (time btn collection and culture)• Hygiene→Sample contaminationJames Bugeza,  J.Bugeza@cgiar.org ILRI c/o Bioversity International P.O. Box 24384, Kampala Uganda+256 392 081 154/155 This document is licensed for use under the Creative Commons Attribution 4.0 International Licence. September 2022.22 September 2022FindingsILRI thanks all donors and organizations which globally support its work through their contributions to the CGIAR Trust Fund.Contribution to Uganda’s livestock development agenda• Sound control strategies based on the epidemiological context will lead to reductionof production losses → ↑Food and nutritional security, household incomes• Increased farm offtake → increased livestock contribution to GDP• Improved occupational and consumer safetySpecies Sample type # Samples collected (%) Preliminary testsCattle Blood for serum, Lymph nodes, Udder and testicular tissue, spleen893 (>100) Standard RBT, Culture tissues from +ve reactors, Biochemical testing of isolates, archive presumptive coloniesShoats As above 937(>100) Modified RBT  + other procedures as aboveSwine As above 579/695(83.3) Standard RBT + other Procedures as aboveHuman Whole blood, Serum 335/461(72.7) Standard RBT, Culture of blood from +ve reactors +  other procedures as aboveBiochemical profile and district of origin of isolatesCL0081 CL0184 CT0289 ST0409 ST0571 SL0601 SL0891 SL0892 CT0887Urease + + + + + + ND ND NDGlucose － － － － ND ND ND ND NDNitrate + + + + ND ND ND ND NDOxidase + + + + + + + + +Catalase + + + + + + + + +H2S － － － － ND ND ND ND NDCitrate － － － － ND ND ND ND NDMethyl red － － － － ND ND ND ND NDAnimal Spp Cattle Cattle Cattle Goat Goat Goat Goat Goat CattleDistrict Kotido Otuke Amudat Kabong Mbarara UD KyankwanziMbarara GombaSerology resultsSpecies #Samples # Positive (%)Cattle 893 52 (5.8)Goats 728 68 (9.3)Sheep 198 16 (8.1)Swine 579 52 (8.9)Human 335 21 (6.2)No Course1 QGIS2 Data analysis with R3 Sanger sequencing4 Systematic literature review5 Scientific communication6 Molecular methods, genomics and bioinformatics for infectious diseases epidemiology7 GIS with R8 Ethics in research9 Gender and researchILRI thanks all donors and organizations which globally support its work through their contributions to the CGIAR Trust Fund.Capacity building of the PhD fellow under BUILD

Epidemiology of brucellosis at the human-livestock interface in Uganda

https://cgspace.cgiar.org/bitstream/10568/122039/1/Brucellosis%20epidemiology.pdf

Epidemiology of brucellosis at the human-livestock interface in Uganda

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