In vivo effects of interferon-Γ and anti-interferon-Γ antibody on the experimentally induced lichenoid tissue reaction

Abstract

We investigated the in vivo effect of recombinant interferon-Γ (IFN-Γ) and tumour necrosis factor Α (TNF-Α) treatment of mice on the development of the delayed-type hypersensitivity (DTH) reaction and lichenoid tissue reaction (LTR) following the local injection of cloned autoreactive T cells. Both the DTH reaction and the LTR were significantly enhanced by pre-treatment with IFN-Γ, but not with TNF-Ã. Induction of class II MHC antigens on keratinocytes was not essential for the enhancement by IFN-Γ. Administration of anti-IFN-Γ antibody reduced the DTH reaction and LTR, although complete inhibition was not observed with our treatment regimen. The ability of IFN-Γ to increase the number of the cloned T cells invading the epidermis in vivo , is in keeping with our previous observation that IFN-Γ treatment of cultured keratinocytes markedly increased the adherence reaction between T cells and keratinocytes in vitro.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74579/1/j.1365-2133.1988.tb03202.x.pd