Gelatin is a heterogeneous mixture of water-soluble proteins. Its broad molecular weight profile may be responsible for the variation in the experimental conditions required for nanoparticle formation. Since this may hinder the application of gelatin nanoparticles, it is important to determine the effect of several factors on the properties and stability of primary dispersions. The phase behaviour of gelatin in ethanol-water binary solvent systems has already been studied using transmittance values. The objective of this project was to use dynamic light scattering and laser Doppler electrophoresis to increase the understanding of the effects of pH and various dilute gelatin concentrations on the size, polydispersity and zeta potential of dispersions of B225 bovine skin gelatin in hydroalcoholic solutions.peer-reviewe
