Chase, R.

Christelova, P.

Guignon, V.

Houwe, I. van den

Irish, B.

Lehrer, K.

Montalvo-Katz, S.

Roux, N.

Ruas, M.

Ríos, C.

Sardos, J.

Irish, Brian M.

Guignon, Valentin

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Monitoring the genetic integrity of Bioversity’s International Musa Germplasm Transit Centre: A foundation for the conservation and use of banana genetic resources Bioversity International is a CGIAR Research Centre. CGIAR is a global research partnership for a food-secure future. www.cgiar.org Bioversity Headquarters Via dei Tre Denari 472/a 00054 Maccarese (Fiumicino) Rome, Italy Tel. (39) 06 61181 Fax. (39) 06 61979661 Email: bioversity@cgiar.org Chase, R1, Ruas, M1, Montalvo-Katz, S1, Irish, B2, Rios, C2, Guignon, V1, Lehrer, K1, Christelová, P3, Sardos, J1 Van den houwe, I4, Roux, N1 1Bioversity International, Montpellier, France, 2United States Department of Agriculture - Tropical Agriculture Research Station, Mayaguez, Puerto Rico, 3Musa Genotyping Centre, Institute of Experimental Botany, Olomouc, Czech Republic, 4Bioversity International, Leuven, Belgium.  An impact study carried out by Bioversity International in 2016 on the use of ITC accessions by Musa collections in the Latin America and Caribbean region showed the importance of well-documented accessions in regional and national collections.   More diverse collections At the Instituto de Investigaciones de Viandas Tropicales (INIVIT) in Cuba, ITC materials were used to test tolerance of varieties to black Sigatoka before it spread to the country. They also received the ITC accession Zanzebar (ITC1471) as part of the IMTP evaluation trial, which is now a common and important variety known as PV 0630.  Training and research At USDA-ARS Tropical Agricultural Research Station the diverse ITC accessions are used in the field and the lab to train students, interns, and Extension Agents from the University of Puerto Rico and the Department of Agriculture. They also produced a catalogue of their diverse field collection using the phenotypic descriptors from the collaborative field verification exercise (photo bottom right).  Production and breeding At the Corporación Bananera Nacional (CORBANA) in Costa Rica, the collection is an important backup resource for farmers in this high banana production country. They are testing disease resistance in their diverse collection, which includes many ITC accessions. Checking the genetic integrity of the ITC banana accessions How better documentation contributes to greater use of PGR www.ars.usda.gov www.crop-diversity.org/mgis The morphological and molecular data have a second purpose: to increase the publicly accessible documentation associated with ITC accessions. The link between the genetic integrity exercise and users of data is the MGIS website (see below), which contains passport data, botanical classification, morpho-taxonomic descriptors, molecular diversity trees, ploidy and digital images currently for more than 4,500 accessions from 21 Musa collections worldwide. How the data adds value to the ITC Results Final conclusions on the genetic integrity of an accession is determined to be either: True to type/subgroup (TTT/TTS) or non-true to type (i.e. off-type (OT), mislabeled (ML) or misclassified (MC)).  As of 2017, determinations have been made for 378 accessions: 310 accessions are true to type/subgroup, and 68 accessions are non-true to type (pie chart below).  The International Musa Germplasm Transit Centre (ITC) is the world’s largest collection of banana germplasm, with more than 1,500 accessions (varieties in a collection) of edible cultivars and wild species, hosted at the Katholieke Universiteit Leuven (KU Leuven), Belgium.  The ITC also serves as a transit centre and safety backup for banana collections worldwide.  The ITC conserves accessions by in vitro storage, cryopreservation and leaf lyophilization. Protocols for seed conservation are currently being developed. Banana germplasm  can be requested by users free of charge via the Musa Germplasm Information System (MGIS). The genetic integrity of available germplasm is verified by a 5 step process (right). Global banana genebank (ITC)   The wealth of data generated from the Genetic Integrity exercise will also be used to investigate other topics of interest to crop genebanks. Genetic stocks of the off-type accessions that were identified through the routine monitoring of the ITC collection will be held in perpetuity for future studies on subjects such as the detection of somaclonal variation at an earlier stage.   Moving further with our partners at USDA-ARS TARS and also at the Agricultural Research Institute (ARI) in Tanzania, we aim to validate the banana cryopreservation method by comparing the ITC accessions that have been held for years in cryopreservation, with plants held in vitro and plants only grown in field. Perspectives 1. ITC accessions in vitro for >10 years  2a. Field Verification at  USDA-ARS TARS Puerto Rico 3. Morphological and molecular data compiled on MGIS dashboard 2b. Ploidy determination and genotyping (SSR) at the Musa Genotyping Centre (MGC) 4. Taxonomic Advisory Group (TAG) expert review 5. Final determination of genetic integrity www.bioversityinternational.org  MGC The plantain Ihitisim (photo left) was found to be off-type in the ITC (photo right). USDA-TARS Puerto Rico R. Chase @Bioversity  I. Van den  Houwe @Bioversity  N. Capozio @Bioversity L. Ibobondji @ CARBAP USDA-TARS Puerto Rico S. Montalvo-Katz @ Bioversity I. Van den  Houwe @Bioversity  

Monitoring the genetic integrity of Bioversity’s International Musa Germplasm Transit Centre: A foundation for the conservation and use of banana genetic resources

https://cgspace.cgiar.org/bitstream/10568/83237/1/Chase_EucarpiaPoster2017.pdf

Monitoring the genetic integrity of Bioversity’s International Musa Germplasm Transit Centre: A foundation for the conservation and use of banana genetic resources

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