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S221T/D225Y mutations of Escherichia coli RecA can transfer RecA from cytoplasm to nucleus in HeLa cells

Abstract

RecA/Rad51家族蛋白在进化上是非常古老而又保守的蛋白家族。大肠杆菌RecA基因在同源重组、DNA修复、SOS途径、突变和细胞分裂中起重要作用。人Rad51基因是大肠杆菌RecA基因的直向同源基因,两者起源于同一个祖先基因。人Rad51基因主要参与DNA的修复和重组,并且与肿瘤发生关系密切。原核细胞与真核细胞最大的区别在于原核细胞没有以核模为界限的细胞核,而蛋白质的入核转运机制是一个十分复杂的生物学过程,需要核孔复合体(NPC)、核定位信号(NLS)、核转运受体、RanGTP蛋白等共同参与。 在HeLa细胞中,人Rad51蛋白定位于细胞核中,相反,作为人Rad51的同源基因,大肠杆菌R...RecA/Rad51 gene family encodes a set of proteins which are ancient and conservative.E.coli RecA gene plays an important role in homology recombination, DNA repair,SOS response, gene mutation and cell division. Human Rad51, an ortholog of bacterial RecA, is essential for homologous recombination and is involved in human tumors. Eukaryocyte is different from prokaryote mainly in nucleus which is enc...学位:理学硕士院系专业:生命科学学院_生物化学与分子生物学学号:2162013115260

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