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Adenosine Monophosphate-Activated Protein Kinase (AMPK) and Calreticulin (CRT) Genes in the Mud Crab, Scylla paramamosain: cDNA Cloning and Profiles under Low Temperature Stress

Abstract

低温胁迫会影响生物体内多个生理过程,如能量代谢和免疫应答等;而生物体内有多种物质参与低温胁迫调节。AMP活化蛋白激酶(AMP-activatedproteinkinase,AMPK)的主要功能为促进ATP产物的积累和减少ATP的消耗,使得机体内积累大量的ATP以供生命活动的能量需要。钙网蛋白(calreticulin,CRT)主要存在于内质网和肌浆网,不仅作为细胞内质网的分子伴侣参与新生蛋白的折叠及调节细胞内Ca2+平衡,而且具有促进细胞黏附、调节免疫应答等重要作用。为探讨这2个基因在拟穴青蟹(Scyllaparamamosain)低温胁迫中的作用,本研究采用RT-PCR、RACE等技术,从拟...Low temperature stress can affect multiple physiological processes, such as energy metabolism and immune response; and various factors in organisms have attended the regulatory response to the cold stress. AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy metabolism. AMPK can switch ATP-generating catabolic pathways and shut off ATP-consuming anabolic processes...学位:理学硕士院系专业:海洋与地球学院_海洋生物学学号:2232013115140

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