为了保持菌体固有的生物活性,使其重复使用,将产酸克雷伯氏菌(HP1,Klebsiella oxytoca)包埋在凝胶中,采用注滴法制备海藻酸钙凝胶球来固定产酸克雷伯氏菌HP1,达到截留菌体产氢的目的.结果表明,选择培养6～8 h的菌体作为海藻酸钙凝胶球包埋的菌体,Tris做培养基中的缓冲盐,当体积质量为5 g/L时,最佳产氢初始pH值为9.0,温度为37℃,采用柱反应器连续产氢7 d,平均产氢速率为3.38×10-2 moL(/L.h)-1.Biological H2-production is considered as an important and economic way to produce hydrogen in the future for energy supply.Ca-alginate gel beads with Klebsiella oxytoca HP1 were prepared by dripping method,so it is easy to keep bacterium and continue to produce hydrogen.The result of experiment showed that choosing the germ body of 6～8 h as the germ fixed by Ca-alginate gel beads and Tris as the buffer the density of which is 5 g/L.The optimal conditions for hydrogen production were: initial pH=9.0,ferment temperature 37 ℃.The average rate of H2-production was achieved as 33.8 mmol/L·h,during a 7days continuous hydrogen production
