目的:优选平糖胶囊b方的微波辅助提取工艺。方法:采用微波辅助提取平糖胶囊b方,以栀子苷提取量为检测指标,选择微波功率、料液比、提取时间、提取次数为考察指标,通过正交试验优选微波辅助提取工艺。采用HPlC测定栀子苷含量,色谱条件为dIAMOnSIlTMrP C18色谱柱(4.6 MMx250 MM,5μM),流动相乙腈-水(11∶89),流速1.0 Ml·MIn-1,检测波长238 nM,柱温室温,进样量20μl。结果:最佳工艺条件为微波功率15 kW,料液比1∶5,提取时间20 MIn,提取数2次,栀子苷平均提取量9.08 Mg·g-1。结论:优选的微波辅助提取工艺具有高效、节能、省时等优点,具有极大的工业化推广价值。Objective: To optimize microwave-assisted extraction technology of Pingtang capsules B.Method: Pingtang capsules B was extracted by microwave assisted technology,with yield of geniposide as index,choosing microwave power, material-liquid ratio, extraction time, extraction times as investigating factors,orthogonal test was adopted to optimize microwave assisted extraction technology.The content of geniposide was determined by HPLC,chromatographic conditions were as follows: DiamonsilTMRP C18column(4.6 mm × 250mm,5 μm),mobile phase of acetonitrile-water(11∶ 89),flow rate 1.0 mL·min- 1,detection wavelength 238nm,column temperature at room temperature,injection volume 20 μL.Result: Optimum extraction technology conditions were as follows: microwave power 15 kW,solid-liquid rate 1∶ 5,extraction time 20 min,extraction 2times; Under these conditions,average yield of geniposide was 9.08 mg·g- 1.Conclusion: Optimized microwave assisted extraction technology had advantages of high efficiency, energy saving and time saving with great application value for industrial production of Pingtang capsules B.厦门市科技局科研项目(3502Z20044013
