利用光纤荧光法实现了吸附于白骨壤(AM)、海漆(EA)、秋茄(kC)、桐花树(AC)和老鼠簕(AI)叶片表面上荧蒽(f lA)的定量测定。所建方法测定吸附于AM、EA、kC、AC和AI叶片表面上f lA的线性范围分别为2.5--500、2.0--600、4.5--1 100、15--600、3.5--450 ng/SPOT,相应的检出限分别为0.91、0.63、1.12、3.52、1.40 ng/SPOT,方法的相对标准偏差小于7.8%(n=15),加标回收率分别为97%--108%、78%--95%、77%--90%、84%--108%和78%--102%。利用所建方法考察了5种红树叶片正反面吸附不同量f lA的信号强度随时间的变化情况。结果表明:在200 M In内,叶片正反面上f lA的信号均发生不同程度的衰减,且反面的信号衰减率大于正面;5种红树叶片正反面对f lA的吸附特性均不同;吸附于叶片正面的f lA多残留在叶片表面,而吸附于叶片反面的f lA易向叶片内部迁移。A fiber optical fluorimetry for determination of fluoranthene(Fla) adsorbed onto the surface of five kinds of mangrove leaves was established.Experimental results showed that the linear dynamic ranges for determination of Fla adsorbed onto Avicennia marina(Am),Excoecarla agallocha(Ea),Kandelia candel(Kc),Aegiceras corniculatum(Ac) and Acanthus ilicifolius(Ai) leaves were 2.5-500,2.0-600,4.5-1 100,15-600 and 3.5-450 ng/spot,with detection limits of 0.91,0.63,1.12,3.52 and 1.40 ng/spot,respectively.The relative standard deviations were less than 7.8%(n=15).The experimental recoveries for Fla adsorbed onto Am,Ea,Kc,Ac and Ai leaves were 97%-108%,78%-95%,77%-90%,84%-108% and 78%-102%,respectively.The variation of fluorescence signals of different amounts of Fla adsorbed onto upper and lower surface of five kinds of mangrove leaves with time were also investigated.The results showed that fluorescence intensities of Fla adsorbed onto two surfaces of mangrove leaves were decreased differently in 200 min,and the fluorescence decrements of Fla adsorbed onto lower surface were all bigger than those from the corresponding upper surface.Both of upper and lower surfaces of the five mangrove species showed different capacities to adsorb Fla.Most of Fla adsorbed on the upper surface of mangrove leaves were remained,while some of the Fla adsorbed onto the lower surface of leaves could be penetrated easily into the inner sides of mangrove leaves.国家自然科学基金资助项目(20777062);教育部博士点基金资助项目(200803840015
