Observation of Life Cycle and Control of Parasite Amyloodinium ocellatum at Cyst Stage

Abstract

自发病的大黄鱼鳃上取得眼点淀粉卵涡鞭虫滋养体,置于凹玻片上连续观察其分裂过程。淀粉卵涡虫包囊的直径为61.3~112.7μM,平均(85.3±16.0)μM。以二分裂的方式分裂,每完成一次分裂细胞数量增加1倍。水温27~29℃时,大多数滋养体经8~12H后进入二细胞期,此后4~10H完成一次分裂。所观察的包囊中,最多的能完成8次分裂,最少的完成2次分裂。不同分裂次数的包囊均孵出涡孢子,滋养体脱落至涡孢子孵出需48~66H。硫酸铜硫酸亚铁合剂可以对大黄鱼苗鳃丝上的眼点淀粉卵涡鞭虫滋养体起到明显的驱除作用。The theronts of parasite Amyloodinium ocellatum were collected from infected large yellow croaker,Larimichthys crocea,and placed on the concave glass for observation of the continuously splitting process.It was found that the theronts had diameter of 61.3-112.7μm,and reproduction with two division ways,one time increase in cell quantity by each completed cell division.Most of the theronts developed into two cell phase in 8-12 h,then completed a division every 4-10 hat water temperature of 27-29℃,with the maximum 8times and the minimum 2times.The spores were hatched from all the theronts with different division frequency in 48-66 h.The obvious removal of the theronts were observed in the gill filaments of the large yellow croaker exposed to mixture of copper sulfate and ferrous sulfate.福建省2012年科学计划区域重大项目(2012N3013

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