The primary analysis of the epitopes of anti-HEV mAbs and the molecular differences between HEV-1 and HEV-4 in their neutralization regions

Abstract

戊型肝炎病毒（HEV）是戊型肝炎的病原体，随着HEV流行病学研究的深入，发现HEV的4个主要基因型存在着明显的易感宿主差异：基因1型（HEV-1）和基因2型（HEV-2）仅分离于人类，能导致大规模的戊肝爆发流行，实验动物目前只成功感染非人灵长类，而基因3型（HEV-3）和基因4型（HEV-4）人畜共患，仅见于小规模流行和临床散发。我们将HEV分为两类：包括HEV-1/2的H（Human）类和包括HEV-3/4的Z（Zoonosis）类。 首先我们通过Westernblot、体外捕获PCR、ELISA阻断实验及合成的多肽库对实验室制备的多株抗HEV单抗的识别表位进行系统的研究，结果发现12株线...Hepatitis E virus (HEV) is the pathogen of hepatitis E. With the increasing knowledge of the molecular epidemiological study, HEV can be classified into two groups according to its compatible host: H (human) group and Z (zoonosis) group. H group contains genotype 1 and genotype 2 HEV isolates which infect human only; Z group contains genotype 3 and genotype 4 HEV isolates which infect both human a...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：20042615