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Constituents research of Cayratia japonica(Thunb.) Gagnep and its activite ingredient research

Abstract

乌蔹莓是我国传统的中药,在我国具有悠久的用药历史,早在《唐本草》中就有了关于其药理作用的介绍。如今,已知其药理活性多样,临床应用广泛,如治疗腰椎间盘推出,促进生长,抗菌抗病毒等,但对于其抗肿瘤活性方面研究较少。本研究对乌蔹莓具有抗肿瘤作用的活性成分进行了初步研究。结果发现,乌蔹莓中含有可以明确杀死肿瘤细胞的活性成分。 本文对乌蔹莓的60%乙醇提取物的大孔吸附树脂60%乙醇和95%乙醇洗脱部分进行了分离,综合运用硅胶柱色谱、ODS柱色谱、凝胶柱色谱、分析型HPLC、制备型HPLC及重结晶等方法从以上两个洗脱部分中分离得到了21个化合物。通过NMR、质谱、熔点及理化性质等对化合物的结构进行了鉴定...Cayratia japonica (Thunb.)Gagnep is a traditional Chinese medicine. It was first introduced by 《Tang Ben Cao》and had been used with long history in our country. Today, it’s diverse pharmacological activity and clinical application is reported on the treatment of lumbar disc, promoting rat growth, antibacterial and antiviral, etc. However, its antitumor activity study is still not concerned. In thi...学位:理学硕士院系专业:生物医学研究院_化学生物学专业学号:3052009115222

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