unknown

Isolation, purification and structure analysis of the polysaccharides from the bio-contorl agent, Peacilomyces ilacinus

Abstract

筛选到一株对枯萎病病原菌-尖孢镰刀菌(Fusariumoxysporum)具有拮抗抑制作用的淡紫拟青霉(Peacilomycesilacinus)菌株NH-PL-03,抗性物质初步筛选结果表明其胞外粗多糖具有抑菌活性。本论文对该菌株的胞内、外多糖进行了提取、纯化研究,并对纯化所得的两个单组分多糖IP-1和EP-1进行了结构分析以及抑菌活性测定。主要研究内容与结果如下: 1、通过形态学和rDNA-ITS序列分析,鉴定出分离保存的菌株为淡紫拟青霉。对其培养特性进行了研究。 2、淡紫拟青霉发酵液的胞内、胞外物质经过醇析法加乙醇3倍即可得到多糖粗提物。经过测试反应验证,该粗提物为多糖,并具有凝胶多...An anti-Fusarium oxysporum activity strain NH-PL-03 of Paecilomyces lilacinus was obtained by screening from the soil in Fuzhou City of Fujian Province in 2003. The results showed that the crude extracelluar polysaccharide of NH-PL-03 strain had antifungal activity to F. oxysporum. In this dissertation, the extraction and purification of polysaccharides from P. lilacinus were conducted. Two monoco...学位:理学硕士院系专业:生命科学学院生物化学与生物技术系_生物化学与分子生物学学号:K20014503

    Similar works