Cell free hemolymph from Haliotis diversicolor supertexta was prepared from fluid collected at 1, 4, 8. 12. 24, 48 96 It after injection with either Escherichia coli, Vibrio parahaemolyticus or 0.9 NaCl solution (control group). The response of selected innate immune parameters (lysozyme, antibacterial activity, alkaline phosphatase, acid phosphatase, phenoloxidase, and superoxide dismutase) was investigated. Results showed that the activities of ACP (Acid Phosphatase) from abalone injected with V. parahaemolyticus were much higher than that of the control group at 24 h after injection. The ALP (Alkaline Phosphatase) activities of abalone challenged with V. parahaemolyticus were significantly higher than those of the control group at 8 h and increased further up to 48 h after the challenge. In contrast, the activities of ALP and ACP in the E. coli-challenged group showed no statistically significant differences at any of the sampling times. The activities of SOD (Superoxide Dismutase) in cell free hemolymph from the V. parahaemolyticus-exposed group were significantly lower than those of the control group at both I h and 24 h, whereas there was no difference in SOD activity observed in the group exposed to E. coli at any of the sampling times. The activities of lysozyme and phenoloxidase in Haliotis diversicolor were relatively low in both control and bacteria-ex posed groups when compared with reports for other invertebrates no significant difference was found between the infected groups and the control for these two parameters, due to the low activities and high individual variance
